Umbelliferone increases the expression of adipocyte-specific genes in 3 T3-L1 adipocyte

Jarinyaporn Naowaboot, Choon Hee Chung, Ran Choi, Patchareewan Pannangpetch

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Umbelliferone (UMB), a natural product of coumarin family, has been shown to reduce blood glucose and to improve lipid profiles in streptozotocin (STZ)-induced diabetic rats. Our objective was to examine the effect of UMB on adipogenesis by investigating its stimulatory effect on lipid accumulation and mRNA expression of adipogenic transcription factors and adipocyte-specific genes in 3T3-L1 preadipocyte culture.An Oil Red Ostaining was used to monitor lipid accumulation, and we found that UMB treatment at concentration range of 10-100μMsignificantly increased lipid accumulation of differentiating 3T3-L1 cells.At the molecular level of adipogenesis, we examined themRNA expression of adipogenic transcription factors, peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c. Those transcription factors were increased by UMB at 10-100/μM. Interestingly, UMB also stimulated the mRNA expression of adipocyte-specific genes, adipocyte fatty acid-binding protein, lipoprotein lipase, fatty acid synthase, fatty acid translocase, and adiponectin. Our findings indicate that the stimulatory effect of UMB on adipocyte differentiation likely occurs through up-regulation of adipogenic transcription factors and downstream adipocyte-specific gene expression.

Original languageEnglish
Pages (from-to)1671-1675
Number of pages5
JournalPhytotherapy Research
Volume28
Issue number11
DOIs
Publication statusPublished - 2014 Jan 1

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7-hydroxycoumarin
Adipocytes
Transcription Factors
Genes
Lipids
Adipogenesis
Sterol Regulatory Element Binding Protein 1
CCAAT-Enhancer-Binding Proteins
3T3-L1 Cells
Fatty Acid Synthases
Fatty Acid-Binding Proteins
Messenger RNA
Peroxisome Proliferator-Activated Receptors
Lipoprotein Lipase
Adiponectin
Streptozocin
Biological Products
Blood Glucose
Oils
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Naowaboot, Jarinyaporn ; Chung, Choon Hee ; Choi, Ran ; Pannangpetch, Patchareewan. / Umbelliferone increases the expression of adipocyte-specific genes in 3 T3-L1 adipocyte. In: Phytotherapy Research. 2014 ; Vol. 28, No. 11. pp. 1671-1675.
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Umbelliferone increases the expression of adipocyte-specific genes in 3 T3-L1 adipocyte. / Naowaboot, Jarinyaporn; Chung, Choon Hee; Choi, Ran; Pannangpetch, Patchareewan.

In: Phytotherapy Research, Vol. 28, No. 11, 01.01.2014, p. 1671-1675.

Research output: Contribution to journalArticle

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