Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors

Aurimas Vyšniauskas, Maryam Qurashi, Nathaniel Gallop, Milan Balaz, Harry L. Anderson, Marina K. Kuimova

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Viscosity and temperature variations in the microscopic world are of paramount importance for diffusion and reactions. Consequently, a plethora of fluorescent probes have evolved over the years to enable fluorescent imaging of both parameters in biological cells. However, the simultaneous effect of both temperature and viscosity on the photophysical behavior of fluorophores is rarely considered, yet unavoidable variations in temperature can lead to significant errors in the readout of viscosity and vice versa. Here we examine the effect of temperature on the photophysical behavior of three classes of viscosity-sensitive fluorophores termed 'molecular rotors'. For each of the fluorophores we decouple the effect of temperature from the effect of viscosity. In the case of the conjugated porphyrin dimer, we demonstrate that, uniquely, simultaneous dual-mode lifetime and intensity measurements of this fluorophore can be used for measuring both viscosity and temperature concurrently.

Original languageEnglish
Pages (from-to)5773-5778
Number of pages6
JournalChemical Science
Volume6
Issue number10
DOIs
Publication statusPublished - 2015 Jul 4

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Fluorophores
Rotors
Viscosity
Temperature
Porphyrins
Fluorescent Dyes
Dimers
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Vyšniauskas, A., Qurashi, M., Gallop, N., Balaz, M., Anderson, H. L., & Kuimova, M. K. (2015). Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors. Chemical Science, 6(10), 5773-5778. https://doi.org/10.1039/c5sc02248g
Vyšniauskas, Aurimas ; Qurashi, Maryam ; Gallop, Nathaniel ; Balaz, Milan ; Anderson, Harry L. ; Kuimova, Marina K. / Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors. In: Chemical Science. 2015 ; Vol. 6, No. 10. pp. 5773-5778.
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Vyšniauskas, A, Qurashi, M, Gallop, N, Balaz, M, Anderson, HL & Kuimova, MK 2015, 'Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors', Chemical Science, vol. 6, no. 10, pp. 5773-5778. https://doi.org/10.1039/c5sc02248g

Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors. / Vyšniauskas, Aurimas; Qurashi, Maryam; Gallop, Nathaniel; Balaz, Milan; Anderson, Harry L.; Kuimova, Marina K.

In: Chemical Science, Vol. 6, No. 10, 04.07.2015, p. 5773-5778.

Research output: Contribution to journalArticle

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AU - Kuimova, Marina K.

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