Unravelling the enhancement of biohydrogen production via adding magnetite nanoparticles and applying electrical energy input

Alsayed Mostafa, Seongwon Im, Young Chae Song, Jong Hun Park, Sang Hyoun Kim, Kyeong Ho Lim, Dong Hoon Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Magnetite nanoparticles (MNP)-caused enhancements for H2 yield (HY) are usually justified based on oxidation-reduction potential (ORP), iron ions (Fe+2/+3) concentration, and enzymatic activity, that is pH-dependent. However, the questions “If pH, ORP, and Fe+2/+3 impacts are excluded, will MNP-caused HY enhancement be still present? and how electrical energy input (EEI) affects HY?” are still unanswered. Herein, control, MNP-supplemented, EEI-applied, FeCl3-, and Na2S-supplemented batches, referred as G1, G2, G3, G5, and G6, respectively were conducted, under fixed pH (6.0 ± 0.1). G5 and G6 targeted quantifying sole impact of Fe+2/+3, and ORP, respectively on HY. G1, G2, G3, G5, and G6 achieved HY values of 1.10 ± 0.05, 1.66 ± 0.07, 1.38 ± 0.06, 1.18 ± 0.04, and 1.16 ± 0.05 mol H2/molhexose, respectively. Neither Fe+2/+3 release nor ORP reduction significantly affected HY. Further, Clostridium amylolyticum dominance was almost similar among G2, and G3. Metabolites flux analysis and functional genes’ prediction highlighted that G2 achieved highest hydrogenase expression and lowest homoacetogenic H2 consumption.

Original languageEnglish
Pages (from-to)40628-40636
Number of pages9
JournalInternational Journal of Hydrogen Energy
Issue number96
Publication statusPublished - 2022 Dec 12

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning ( NRF-2021R1A2C2013341 ) and the research grant of the Kongju National University in 2017.

Publisher Copyright:
© 2021 Hydrogen Energy Publications LLC

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


Dive into the research topics of 'Unravelling the enhancement of biohydrogen production via adding magnetite nanoparticles and applying electrical energy input'. Together they form a unique fingerprint.

Cite this