Up-regulation of Idh3α causes reduction of neuronal differentiation in PC12 cells

Sun A. Cho, Min Ji Seo, Je Yeong Ko, Jung Hee Shim, Jin Yoo, Jung Hee Kim, Se Yoon Kim, Na Kyung Ryu, Eun Young Park, Han Woong Lee, Yeon Su Lee, Young Yil Bahk, Jong Hoon Park

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Abstract

The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate Idh3α expression by the time course. To identify the function of Idh3α in neuronal differentiation stage, the transfection of Idh3α to PC12 cells was performed. As a result, we proved that up-regulation of Idh3α causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that Idh3α plays a role to the neuronal differentiation.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalBMB reports
Volume43
Issue number5
DOIs
Publication statusPublished - 2010 May

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Cho, S. A., Seo, M. J., Ko, J. Y., Shim, J. H., Yoo, J., Kim, J. H., Kim, S. Y., Ryu, N. K., Park, E. Y., Lee, H. W., Lee, Y. S., Bahk, Y. Y., & Park, J. H. (2010). Up-regulation of Idh3α causes reduction of neuronal differentiation in PC12 cells. BMB reports, 43(5), 369-374. https://doi.org/10.5483/BMBRep.2010.43.5.369