Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells

Hyun Kyu Kang, Ha Young Lee, Youl Nam Lee, Eun Jin Jo, Jung Im Kim, Gi Young Kim, Yeong Min Park, Do Sik Min, Akihiko Yano, Jong Young Kwak, Yoe Sik Bae

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Abstract

Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.

Original languageEnglish
Pages (from-to)911-920
Number of pages10
JournalInternational Immunopharmacology
Volume4
Issue number7
DOIs
Publication statusPublished - 2004 Jul

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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    Kang, H. K., Lee, H. Y., Lee, Y. N., Jo, E. J., Kim, J. I., Kim, G. Y., Park, Y. M., Min, D. S., Yano, A., Kwak, J. Y., & Bae, Y. S. (2004). Up-regulation of phospholipase Cγ1 and phospholipase D during the differentiation of human monocytes to dendritic cells. International Immunopharmacology, 4(7), 911-920. https://doi.org/10.1016/j.intimp.2004.04.001