Phospholipase C (PLC)γ and phospholipase D (PLD) play pivotal roles in the signal transduction required for various cellular responses, including cell proliferation and differentiation. Dendritic cells (DCs), which are professional antigen-presenting cells, can be generated from human monocytes by stimulating the cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). We investigated whether PLCγ and PLD expression levels can be changed during the differentiation of the human monocytes into DCs. The enzymatic activity and protein level of PLC γ1 were significantly increased in the human monocyte-derived DCs by GM-CSF/IL-4, but the protein levels of PLC γ2 were unaltered. Moreover, the enzymatic activity and protein level of PLD1b and PLD2 were up-regulated during the differentiation of human monocytes to DCs, but those of PLD1a were not changed. A higher phagocytic activity of DCs was found to be correlated with the up-regulations of PLCγ1 and PLD, and the phagocytic activity of DCs was inhibited by a PLC-specific inhibitor (U73122) and by a phosphatidic acid acceptor (n-butanol), but to be increased by phosphatidic acid. Thus, suggesting that PLC and PLD participate in the process. This study suggests that the up-regulations of PLCγ1 and PLD are accompanied by the differentiation of monocytes into DCs, which results in increased phagocytic activity.
Bibliographical noteFunding Information:
This work was supported by the Korea Science and Engineering Foundation through the Medical Science and Engineering Research Center for Cancer Molecular Therapy at Dong-A University and by a grant 02-PJI-PG10-20706-0001 from the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy