Upper Particle Size Limit for High-Speed Analysis by Sedimentation/Steric Field-Flow Fractionation in Thin Channels

Myeong Hee Moon, J. Calvin Giddings

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This work describes some of the limitations encountered when sedimentation/steric field-flow fractionation is used to separate and analyze particles whose diameters approach a substantial fraction of the thickness of the FFF channel. Results obtained for a thin (127 μm thick) channel indicate a departure from linear calibration for particle diameters that approach 25-30% of the channel thickness and a substantial loss of selectivity for diameters at 50% of the channel thickness. These results are explained by a simple theoretical model. In addition, our measurements show that the larger particles (in this case polystyrene latex beads of 60 μm. diameter) are subject to peak distortion and sample loss unless high flowrates and low field strengths are employed.

Original languageEnglish
Pages (from-to)1072-1077
Number of pages6
JournalIndustrial and Engineering Chemistry Research
Volume35
Issue number4
DOIs
Publication statusPublished - 1996 Jan 1

Fingerprint

Fractionation
Sedimentation
Flow fields
Particle size
Latexes
Particles (particulate matter)
Polystyrenes
Calibration
styrofoam

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

@article{ff3c41183b434a5bb3fd2de6f13c27b2,
title = "Upper Particle Size Limit for High-Speed Analysis by Sedimentation/Steric Field-Flow Fractionation in Thin Channels",
abstract = "This work describes some of the limitations encountered when sedimentation/steric field-flow fractionation is used to separate and analyze particles whose diameters approach a substantial fraction of the thickness of the FFF channel. Results obtained for a thin (127 μm thick) channel indicate a departure from linear calibration for particle diameters that approach 25-30{\%} of the channel thickness and a substantial loss of selectivity for diameters at 50{\%} of the channel thickness. These results are explained by a simple theoretical model. In addition, our measurements show that the larger particles (in this case polystyrene latex beads of 60 μm. diameter) are subject to peak distortion and sample loss unless high flowrates and low field strengths are employed.",
author = "Moon, {Myeong Hee} and Giddings, {J. Calvin}",
year = "1996",
month = "1",
day = "1",
doi = "10.1021/ie9503297",
language = "English",
volume = "35",
pages = "1072--1077",
journal = "Industrial & Engineering Chemistry Product Research and Development",
issn = "0888-5885",
publisher = "American Chemical Society",
number = "4",

}

Upper Particle Size Limit for High-Speed Analysis by Sedimentation/Steric Field-Flow Fractionation in Thin Channels. / Moon, Myeong Hee; Giddings, J. Calvin.

In: Industrial and Engineering Chemistry Research, Vol. 35, No. 4, 01.01.1996, p. 1072-1077.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Upper Particle Size Limit for High-Speed Analysis by Sedimentation/Steric Field-Flow Fractionation in Thin Channels

AU - Moon, Myeong Hee

AU - Giddings, J. Calvin

PY - 1996/1/1

Y1 - 1996/1/1

N2 - This work describes some of the limitations encountered when sedimentation/steric field-flow fractionation is used to separate and analyze particles whose diameters approach a substantial fraction of the thickness of the FFF channel. Results obtained for a thin (127 μm thick) channel indicate a departure from linear calibration for particle diameters that approach 25-30% of the channel thickness and a substantial loss of selectivity for diameters at 50% of the channel thickness. These results are explained by a simple theoretical model. In addition, our measurements show that the larger particles (in this case polystyrene latex beads of 60 μm. diameter) are subject to peak distortion and sample loss unless high flowrates and low field strengths are employed.

AB - This work describes some of the limitations encountered when sedimentation/steric field-flow fractionation is used to separate and analyze particles whose diameters approach a substantial fraction of the thickness of the FFF channel. Results obtained for a thin (127 μm thick) channel indicate a departure from linear calibration for particle diameters that approach 25-30% of the channel thickness and a substantial loss of selectivity for diameters at 50% of the channel thickness. These results are explained by a simple theoretical model. In addition, our measurements show that the larger particles (in this case polystyrene latex beads of 60 μm. diameter) are subject to peak distortion and sample loss unless high flowrates and low field strengths are employed.

UR - http://www.scopus.com/inward/record.url?scp=0030128649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030128649&partnerID=8YFLogxK

U2 - 10.1021/ie9503297

DO - 10.1021/ie9503297

M3 - Article

AN - SCOPUS:0030128649

VL - 35

SP - 1072

EP - 1077

JO - Industrial & Engineering Chemistry Product Research and Development

JF - Industrial & Engineering Chemistry Product Research and Development

SN - 0888-5885

IS - 4

ER -