Upregulation of CD47 in regulatory T cells in atopic dermatitis

Nara Lee, Jung U. Shin, Shan Jin, Ki Na Yun, Jin Young Kim, Chang Ook Park, Seo Hyeong Kim, Ji Yeon Noh, Kwanghoon Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: Regulatory T (Treg) cells are key modulators in the immune system. Recent studies have shown that atopic dermatitis (AD) patients have higher numbers of Treg cells; however, little is known about the specific phenotype and function of Treg cells in AD. Materials and Methods: To identify differentially expressed proteins in peripheral induced Treg cells in AD and naturally derived Treg cells in normal controls, CD4 + CD25 + Treg cells were isolated from thymus tissue of normal mice and the spleens of AD mice. Membrane proteins were extracted, and quantitative proteomics labeling with Tandem Mass Tags (TMT) was performed, followed by one-dimensional liquid chromatography/tandem mass spectrometry analysis. Results: Using TMT labeling, we identified 510 proteins, including 63 membrane proteins and 16 plasma membrane proteins. CD47 was one of the upregulated proteins in Treg cells in AD spleens. Although CD47 was expressed in all CD4 + and CD8 + T cells, a significantly higher expression of CD47 was observed in the Treg cells of AD mice and AD patients than in those of normal mice and healthy controls. Furthermore, Treg cells from the spleen showed a significantly higher expression of CD47 than those from the thymus. Conclusion: We found that CD47 is highly expressed in the Treg cells of AD mice, particularly in the spleen. Based on our results, we propose that CD47 high Treg cells are likely induced Treg cells and that upregulated CD47 in the Treg cells of AD patients may play a role in the increased population of Treg cells in AD.

Original languageEnglish
Pages (from-to)1435-1445
Number of pages11
JournalYonsei medical journal
Volume57
Issue number6
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Regulatory T-Lymphocytes
Atopic Dermatitis
Up-Regulation
Spleen
Membrane Proteins
Thymus Gland
Proteins
Tandem Mass Spectrometry
Liquid Chromatography
Proteomics
Blood Proteins
Immune System

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, N., Shin, J. U., Jin, S., Yun, K. N., Kim, J. Y., Park, C. O., ... Lee, K. (2016). Upregulation of CD47 in regulatory T cells in atopic dermatitis. Yonsei medical journal, 57(6), 1435-1445. https://doi.org/10.3349/ymj.2016.57.6.1435
Lee, Nara ; Shin, Jung U. ; Jin, Shan ; Yun, Ki Na ; Kim, Jin Young ; Park, Chang Ook ; Kim, Seo Hyeong ; Noh, Ji Yeon ; Lee, Kwanghoon. / Upregulation of CD47 in regulatory T cells in atopic dermatitis. In: Yonsei medical journal. 2016 ; Vol. 57, No. 6. pp. 1435-1445.
@article{da2ee6e02a514d3d8fdd456f49d815c6,
title = "Upregulation of CD47 in regulatory T cells in atopic dermatitis",
abstract = "Purpose: Regulatory T (Treg) cells are key modulators in the immune system. Recent studies have shown that atopic dermatitis (AD) patients have higher numbers of Treg cells; however, little is known about the specific phenotype and function of Treg cells in AD. Materials and Methods: To identify differentially expressed proteins in peripheral induced Treg cells in AD and naturally derived Treg cells in normal controls, CD4 + CD25 + Treg cells were isolated from thymus tissue of normal mice and the spleens of AD mice. Membrane proteins were extracted, and quantitative proteomics labeling with Tandem Mass Tags (TMT) was performed, followed by one-dimensional liquid chromatography/tandem mass spectrometry analysis. Results: Using TMT labeling, we identified 510 proteins, including 63 membrane proteins and 16 plasma membrane proteins. CD47 was one of the upregulated proteins in Treg cells in AD spleens. Although CD47 was expressed in all CD4 + and CD8 + T cells, a significantly higher expression of CD47 was observed in the Treg cells of AD mice and AD patients than in those of normal mice and healthy controls. Furthermore, Treg cells from the spleen showed a significantly higher expression of CD47 than those from the thymus. Conclusion: We found that CD47 is highly expressed in the Treg cells of AD mice, particularly in the spleen. Based on our results, we propose that CD47 high Treg cells are likely induced Treg cells and that upregulated CD47 in the Treg cells of AD patients may play a role in the increased population of Treg cells in AD.",
author = "Nara Lee and Shin, {Jung U.} and Shan Jin and Yun, {Ki Na} and Kim, {Jin Young} and Park, {Chang Ook} and Kim, {Seo Hyeong} and Noh, {Ji Yeon} and Kwanghoon Lee",
year = "2016",
month = "11",
day = "1",
doi = "10.3349/ymj.2016.57.6.1435",
language = "English",
volume = "57",
pages = "1435--1445",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "6",

}

Lee, N, Shin, JU, Jin, S, Yun, KN, Kim, JY, Park, CO, Kim, SH, Noh, JY & Lee, K 2016, 'Upregulation of CD47 in regulatory T cells in atopic dermatitis', Yonsei medical journal, vol. 57, no. 6, pp. 1435-1445. https://doi.org/10.3349/ymj.2016.57.6.1435

Upregulation of CD47 in regulatory T cells in atopic dermatitis. / Lee, Nara; Shin, Jung U.; Jin, Shan; Yun, Ki Na; Kim, Jin Young; Park, Chang Ook; Kim, Seo Hyeong; Noh, Ji Yeon; Lee, Kwanghoon.

In: Yonsei medical journal, Vol. 57, No. 6, 01.11.2016, p. 1435-1445.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Upregulation of CD47 in regulatory T cells in atopic dermatitis

AU - Lee, Nara

AU - Shin, Jung U.

AU - Jin, Shan

AU - Yun, Ki Na

AU - Kim, Jin Young

AU - Park, Chang Ook

AU - Kim, Seo Hyeong

AU - Noh, Ji Yeon

AU - Lee, Kwanghoon

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Purpose: Regulatory T (Treg) cells are key modulators in the immune system. Recent studies have shown that atopic dermatitis (AD) patients have higher numbers of Treg cells; however, little is known about the specific phenotype and function of Treg cells in AD. Materials and Methods: To identify differentially expressed proteins in peripheral induced Treg cells in AD and naturally derived Treg cells in normal controls, CD4 + CD25 + Treg cells were isolated from thymus tissue of normal mice and the spleens of AD mice. Membrane proteins were extracted, and quantitative proteomics labeling with Tandem Mass Tags (TMT) was performed, followed by one-dimensional liquid chromatography/tandem mass spectrometry analysis. Results: Using TMT labeling, we identified 510 proteins, including 63 membrane proteins and 16 plasma membrane proteins. CD47 was one of the upregulated proteins in Treg cells in AD spleens. Although CD47 was expressed in all CD4 + and CD8 + T cells, a significantly higher expression of CD47 was observed in the Treg cells of AD mice and AD patients than in those of normal mice and healthy controls. Furthermore, Treg cells from the spleen showed a significantly higher expression of CD47 than those from the thymus. Conclusion: We found that CD47 is highly expressed in the Treg cells of AD mice, particularly in the spleen. Based on our results, we propose that CD47 high Treg cells are likely induced Treg cells and that upregulated CD47 in the Treg cells of AD patients may play a role in the increased population of Treg cells in AD.

AB - Purpose: Regulatory T (Treg) cells are key modulators in the immune system. Recent studies have shown that atopic dermatitis (AD) patients have higher numbers of Treg cells; however, little is known about the specific phenotype and function of Treg cells in AD. Materials and Methods: To identify differentially expressed proteins in peripheral induced Treg cells in AD and naturally derived Treg cells in normal controls, CD4 + CD25 + Treg cells were isolated from thymus tissue of normal mice and the spleens of AD mice. Membrane proteins were extracted, and quantitative proteomics labeling with Tandem Mass Tags (TMT) was performed, followed by one-dimensional liquid chromatography/tandem mass spectrometry analysis. Results: Using TMT labeling, we identified 510 proteins, including 63 membrane proteins and 16 plasma membrane proteins. CD47 was one of the upregulated proteins in Treg cells in AD spleens. Although CD47 was expressed in all CD4 + and CD8 + T cells, a significantly higher expression of CD47 was observed in the Treg cells of AD mice and AD patients than in those of normal mice and healthy controls. Furthermore, Treg cells from the spleen showed a significantly higher expression of CD47 than those from the thymus. Conclusion: We found that CD47 is highly expressed in the Treg cells of AD mice, particularly in the spleen. Based on our results, we propose that CD47 high Treg cells are likely induced Treg cells and that upregulated CD47 in the Treg cells of AD patients may play a role in the increased population of Treg cells in AD.

UR - http://www.scopus.com/inward/record.url?scp=84986903132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986903132&partnerID=8YFLogxK

U2 - 10.3349/ymj.2016.57.6.1435

DO - 10.3349/ymj.2016.57.6.1435

M3 - Article

VL - 57

SP - 1435

EP - 1445

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 6

ER -

Lee N, Shin JU, Jin S, Yun KN, Kim JY, Park CO et al. Upregulation of CD47 in regulatory T cells in atopic dermatitis. Yonsei medical journal. 2016 Nov 1;57(6):1435-1445. https://doi.org/10.3349/ymj.2016.57.6.1435