Upregulation of phospholipase D1 in the spinal cords of rats with clip compression injury

Kyungsook Jung, Do Sik Min, Ki Bum Sim, Meejung Ahn, Hwanglyong Kim, Jongtae Cheong, Taekyun Shin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This study examined phospholipase D 1 (PLD1) expression in the central nervous system following clip compression spinal cord injury (SCI) in Sprague-Dawley rats. After inducing SCI with a vascular clip, the expression of PLD1 in the affected spinal cord was analyzed by Western blot and immunohistochemistry. Western blot analysis showed that the expression of PLD1 gradually increased in the spinal cord on days 0.5, 1, 2, and 4 post injury. Immunohistochemistry showed that some cells, including neurons, astrocytes, and some inflammatory cells, were positive for PLD1 in the lesions at days 1 and 2 post injury. At day 4, the number of PLD1-positive cells in SCI lesions increased, largely matching the increases in ED1-positive macrophages and glial fibrillary acidic protein-positive astrocytes. At this time, macrophages expressed proliferating cell nuclear antigen in addition to PLD1. These results suggest that PLD1 expression is increased in injured spinal cords, and might be involved in the activation and proliferation of macrophages and astrocytes in SCI.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalNeuroscience Letters
Volume336
Issue number2
DOIs
Publication statusPublished - 2003 Jan 16

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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