Use of an artificial antigen containing the 3,6-di-O-methyl-β-D-glucopyranosyl epitope for the serodiagnosis of leprosy

S. N. Cho, T. Fujiwara, S. W. Hunter, T. H. Rea, R. H. Gelber, P. J. Brennan

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55 Citations (Scopus)

Abstract

The coupling of synthetic 3,6-di-O-methyl-β-D-glucopyranosyl-(1→4)-2,3-di-O-methyl-α-L-rhamnopyra nose, the hapten determinant of phenolic glycolipid I from Mycobacterium leprae, to bovine serum albumin (BSA) by reductive amination produced the antigen ε-N-1-[1-deoxy-2,3-di-O-methyl-4-O-(3',6'-di-O-methyl-β-D-glucopyranosyl )-rhamnitol]-lysyl-BSA, which proved highly sensitive in ELISA and showed good concordance with the native glycolipid in analysis of serum samples from 223 leprosy patients. Conjugates prepared from 6-O-methyl-β-D-glucopyranosyl- or β-D-glucopyranosyl-containing disaccharides were inactive and those containing noncyclic 3,6-di-O-methyl-glucitol showed little activity. Thus 3,6-di-O-methyl-β-D-glucopyranose in its cyclic hemiacetal form is necessary for binding anti-glycolipid IgM from leprosy patients. Analysis of serum samples from healthy subjects showed a false-positive rate of 2.4% (four of 169) against the glycolipid and 3.6% (six of 169) against the glycoconjugate. Comparable figures for samples of sera of tuberculosis patients were 3.0% (two of 66) and 9.0% (six of 66), respectively. Alternative synthesizing strategies may diminish this cross-reactivity. The prospects of a fully synthetic specific antigen for the worldwide serodiagnosis of leprosy look promising.

Original languageEnglish
Pages (from-to)311-322
Number of pages12
JournalJournal of Infectious Diseases
Volume150
Issue number3
DOIs
Publication statusPublished - 1984 Jan 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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