Use of Contrast Enhancement and High-Resolution 3D Black-Blood MRI to Identify Inflammation in Atherosclerosis

Jin Hur, Jaeseok Park, Young Jin Kim, Hye Jeong Lee, Hyo Sup Shim, Kyu Ok Choe, Byoung Wook Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives We investigated the contributing factors for plaque enhancement and examined the relationships between regional contrast enhancement and the inflammatory activity of atherosclerotic plaques in an experimental rabbit model using contrast-enhanced high-resolution 3-dimensional (3D) black-blood magnetic resonance imaging (MRI) in comparison with histopathologic analysis. Background Inflammation plays a critical role in plaque initiation, progression, and disruption. As such, inflammation represents an emerging target for the treatment of atherosclerosis. MRI findings suggest that contrast agentinduced signal enhancement is associated with the degree of macrophage infiltration and neovessels that can be detected in plaque. Methods Ten atherosclerotic rabbits and 3 normal control rabbits underwent high-resolution 3D contrast-enhanced black-blood MRI. Magnetic resonance images and the corresponding histopathologic sections were divided into 4 quadrants. Plaque composition was analyzed for each quadrant according to histopathologic criteria (percent of lipid-rich, fibrous, macrophage area and microvessel density) and imaging criteria (enhancement ratio [ER], ER = signal intensitypost/signal intensitypre). Multiple linear regression analysis was performed to determine independent factors for plaque enhancement. Results A total of 62 noncalcified plaques (n = 248; 156 lipid-rich quadrants and 92 fibrous quadrants) were identified based on histopathologic analysis. Mean ER values were significantly higher in atherosclerotic vessel walls than in normal vessel walls (2.03 ± 0.25 vs. 1.58 ± 0.15; p = 0.017). The mean ER values were significantly higher in lipid-rich quadrants compared with the fibrous quadrants (2.14 ± 0.31 vs. 1.84 ± 0.21; p = 0.001). Mean ER values were significantly higher in macrophage-rich plaques compared with the macrophage-poor plaques (2.21 ± 0.28 vs. 1.81 ± 0.22; p = 0.001). Using multiple regression analysis, macrophage area and microvessel density were associated independently with ER values that reflected plaque enhancement (p < 0.001). Conclusions Contrast-enhanced high-resolution 3D black-blood MRI may be an efficient method to detect plaque inflammation.

Original languageEnglish
Pages (from-to)1127-1135
Number of pages9
JournalJACC: Cardiovascular Imaging
Volume3
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

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Atherosclerosis
Macrophages
Magnetic Resonance Imaging
Inflammation
Rabbits
Microvessels
Lipids
Regression Analysis
Atherosclerotic Plaques
Linear Models
Theoretical Models
Magnetic Resonance Spectroscopy

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Hur, Jin ; Park, Jaeseok ; Kim, Young Jin ; Lee, Hye Jeong ; Shim, Hyo Sup ; Choe, Kyu Ok ; Choi, Byoung Wook. / Use of Contrast Enhancement and High-Resolution 3D Black-Blood MRI to Identify Inflammation in Atherosclerosis. In: JACC: Cardiovascular Imaging. 2010 ; Vol. 3, No. 11. pp. 1127-1135.
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title = "Use of Contrast Enhancement and High-Resolution 3D Black-Blood MRI to Identify Inflammation in Atherosclerosis",
abstract = "Objectives We investigated the contributing factors for plaque enhancement and examined the relationships between regional contrast enhancement and the inflammatory activity of atherosclerotic plaques in an experimental rabbit model using contrast-enhanced high-resolution 3-dimensional (3D) black-blood magnetic resonance imaging (MRI) in comparison with histopathologic analysis. Background Inflammation plays a critical role in plaque initiation, progression, and disruption. As such, inflammation represents an emerging target for the treatment of atherosclerosis. MRI findings suggest that contrast agentinduced signal enhancement is associated with the degree of macrophage infiltration and neovessels that can be detected in plaque. Methods Ten atherosclerotic rabbits and 3 normal control rabbits underwent high-resolution 3D contrast-enhanced black-blood MRI. Magnetic resonance images and the corresponding histopathologic sections were divided into 4 quadrants. Plaque composition was analyzed for each quadrant according to histopathologic criteria (percent of lipid-rich, fibrous, macrophage area and microvessel density) and imaging criteria (enhancement ratio [ER], ER = signal intensitypost/signal intensitypre). Multiple linear regression analysis was performed to determine independent factors for plaque enhancement. Results A total of 62 noncalcified plaques (n = 248; 156 lipid-rich quadrants and 92 fibrous quadrants) were identified based on histopathologic analysis. Mean ER values were significantly higher in atherosclerotic vessel walls than in normal vessel walls (2.03 ± 0.25 vs. 1.58 ± 0.15; p = 0.017). The mean ER values were significantly higher in lipid-rich quadrants compared with the fibrous quadrants (2.14 ± 0.31 vs. 1.84 ± 0.21; p = 0.001). Mean ER values were significantly higher in macrophage-rich plaques compared with the macrophage-poor plaques (2.21 ± 0.28 vs. 1.81 ± 0.22; p = 0.001). Using multiple regression analysis, macrophage area and microvessel density were associated independently with ER values that reflected plaque enhancement (p < 0.001). Conclusions Contrast-enhanced high-resolution 3D black-blood MRI may be an efficient method to detect plaque inflammation.",
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Use of Contrast Enhancement and High-Resolution 3D Black-Blood MRI to Identify Inflammation in Atherosclerosis. / Hur, Jin; Park, Jaeseok; Kim, Young Jin; Lee, Hye Jeong; Shim, Hyo Sup; Choe, Kyu Ok; Choi, Byoung Wook.

In: JACC: Cardiovascular Imaging, Vol. 3, No. 11, 01.11.2010, p. 1127-1135.

Research output: Contribution to journalArticle

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T1 - Use of Contrast Enhancement and High-Resolution 3D Black-Blood MRI to Identify Inflammation in Atherosclerosis

AU - Hur, Jin

AU - Park, Jaeseok

AU - Kim, Young Jin

AU - Lee, Hye Jeong

AU - Shim, Hyo Sup

AU - Choe, Kyu Ok

AU - Choi, Byoung Wook

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N2 - Objectives We investigated the contributing factors for plaque enhancement and examined the relationships between regional contrast enhancement and the inflammatory activity of atherosclerotic plaques in an experimental rabbit model using contrast-enhanced high-resolution 3-dimensional (3D) black-blood magnetic resonance imaging (MRI) in comparison with histopathologic analysis. Background Inflammation plays a critical role in plaque initiation, progression, and disruption. As such, inflammation represents an emerging target for the treatment of atherosclerosis. MRI findings suggest that contrast agentinduced signal enhancement is associated with the degree of macrophage infiltration and neovessels that can be detected in plaque. Methods Ten atherosclerotic rabbits and 3 normal control rabbits underwent high-resolution 3D contrast-enhanced black-blood MRI. Magnetic resonance images and the corresponding histopathologic sections were divided into 4 quadrants. Plaque composition was analyzed for each quadrant according to histopathologic criteria (percent of lipid-rich, fibrous, macrophage area and microvessel density) and imaging criteria (enhancement ratio [ER], ER = signal intensitypost/signal intensitypre). Multiple linear regression analysis was performed to determine independent factors for plaque enhancement. Results A total of 62 noncalcified plaques (n = 248; 156 lipid-rich quadrants and 92 fibrous quadrants) were identified based on histopathologic analysis. Mean ER values were significantly higher in atherosclerotic vessel walls than in normal vessel walls (2.03 ± 0.25 vs. 1.58 ± 0.15; p = 0.017). The mean ER values were significantly higher in lipid-rich quadrants compared with the fibrous quadrants (2.14 ± 0.31 vs. 1.84 ± 0.21; p = 0.001). Mean ER values were significantly higher in macrophage-rich plaques compared with the macrophage-poor plaques (2.21 ± 0.28 vs. 1.81 ± 0.22; p = 0.001). Using multiple regression analysis, macrophage area and microvessel density were associated independently with ER values that reflected plaque enhancement (p < 0.001). Conclusions Contrast-enhanced high-resolution 3D black-blood MRI may be an efficient method to detect plaque inflammation.

AB - Objectives We investigated the contributing factors for plaque enhancement and examined the relationships between regional contrast enhancement and the inflammatory activity of atherosclerotic plaques in an experimental rabbit model using contrast-enhanced high-resolution 3-dimensional (3D) black-blood magnetic resonance imaging (MRI) in comparison with histopathologic analysis. Background Inflammation plays a critical role in plaque initiation, progression, and disruption. As such, inflammation represents an emerging target for the treatment of atherosclerosis. MRI findings suggest that contrast agentinduced signal enhancement is associated with the degree of macrophage infiltration and neovessels that can be detected in plaque. Methods Ten atherosclerotic rabbits and 3 normal control rabbits underwent high-resolution 3D contrast-enhanced black-blood MRI. Magnetic resonance images and the corresponding histopathologic sections were divided into 4 quadrants. Plaque composition was analyzed for each quadrant according to histopathologic criteria (percent of lipid-rich, fibrous, macrophage area and microvessel density) and imaging criteria (enhancement ratio [ER], ER = signal intensitypost/signal intensitypre). Multiple linear regression analysis was performed to determine independent factors for plaque enhancement. Results A total of 62 noncalcified plaques (n = 248; 156 lipid-rich quadrants and 92 fibrous quadrants) were identified based on histopathologic analysis. Mean ER values were significantly higher in atherosclerotic vessel walls than in normal vessel walls (2.03 ± 0.25 vs. 1.58 ± 0.15; p = 0.017). The mean ER values were significantly higher in lipid-rich quadrants compared with the fibrous quadrants (2.14 ± 0.31 vs. 1.84 ± 0.21; p = 0.001). Mean ER values were significantly higher in macrophage-rich plaques compared with the macrophage-poor plaques (2.21 ± 0.28 vs. 1.81 ± 0.22; p = 0.001). Using multiple regression analysis, macrophage area and microvessel density were associated independently with ER values that reflected plaque enhancement (p < 0.001). Conclusions Contrast-enhanced high-resolution 3D black-blood MRI may be an efficient method to detect plaque inflammation.

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