Use of Dimethyl Pimelimidate with Microfluidic System for Nucleic Acids Extraction without Electricity

Choong Eun Jin, Tae Yoon Lee, Bonhan Koo, Kyung Chul Choi, Suhwan Chang, Se Yoon Park, Ji Yeun Kim, Sung Han Kim, Yong Shin

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The isolation of nucleic acids in the lab on a chip is crucial to achieve the maximal effectiveness of point-of-care testing for detection in clinical applications. Here, we report on the use of a simple and versatile single-channel microfluidic platform that combines dimethyl pimelimidate (DMP) for nucleic acids (both RNA and DNA) extraction without electricity using a thin-film system. The system is based on the adaption of DMP into nonchaotropic-based nucleic acids and the capture of reagents into a low-cost thin-film platform for use as a microfluidic total analysis system, which can be utilized for sample processing in clinical diagnostics. Moreover, we assessed the use of the DMP system for the extraction of nucleic acids from various samples, including mammalian cells, bacterial cells, and viruses from human disease, and we also confirmed that the quality and quantity of the nucleic acids extracted were sufficient to allow for the robust detection of biomarkers and/or pathogens in downstream analysis. Furthermore, this DMP system does not require any instruments and electricity, and has improved time efficiency, portability, and affordability. Thus, we believe that the DMP system may change the paradigm of sample processing in clinical diagnostics.

Original languageEnglish
Pages (from-to)7502-7510
Number of pages9
JournalAnalytical Chemistry
Volume89
Issue number14
DOIs
Publication statusPublished - 2017 Jul 18

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI16C-0272-010016).

Publisher Copyright:
© 2017 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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