Use of hydrogel microstructures as templates for protein immobilization

Won G. Koh, Nylin Kim Dae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, protein micropatterns were created on the surface of three-dimensional hydrogel microstructures. Poly(ethylene glycol)(PEG)-based hydrogel microstructures were fabricated on a glass substrate using a poly(dimethylsiloxane) (PDMS) replica as a molding insert and photolithography. The lateral dimension and height of the hydrogel microstructures were easily controlled by the feature size of the photomask and depth of the PDMS replica, respectively. Bovine serum albumin (BSA), a model protein, was covalently immobilized to the surface of the hydrogel microstructure via a 5-azidonitrobenzoyloxy N-hydroxysuccinimide bifunctional linker, which has a phenyl azide group and a protein-binding N-hydroxysuccinimide group on either end. The immobilization of BSA on the PEG hydrogel surface was demonstrated with XPS by confirming the formation of a new nitrogen peak, and the selective immobilization of fluorescent-labeled BSA on the outer region of the three-dimensional hydrogel micropattern was demonstrated by fluorescence. A hydrogel microstructure could immobilize two different enzymes separately, and sequential bienzymatic reaction was demonstrated by reacting glucose and Amplex Red with a hydrogel microstructure where glucose oxidase (GOX) was immobilized on the surface and peroxidase (POD) was encapsulated.

Original languageEnglish
Title of host publicationResponsive Biomaterials for Biomedical Applications
Pages60-65
Number of pages6
Volume1095
Publication statusPublished - 2008 Dec 1
Event2008 MRS Spring Meeting - San Francisco, CA, United States
Duration: 2008 Mar 242008 Mar 28

Other

Other2008 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period08/3/2408/3/28

Fingerprint

Hydrogel
immobilization
Hydrogels
templates
proteins
Proteins
microstructure
Microstructure
albumins
serums
Bovine Serum Albumin
Polyethylene glycols
replicas
glucose
Polydimethylsiloxane
photomasks
oxidase
photolithography
inserts
Photomasks

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Koh, W. G., & Dae, N. K. (2008). Use of hydrogel microstructures as templates for protein immobilization. In Responsive Biomaterials for Biomedical Applications (Vol. 1095, pp. 60-65)
Koh, Won G. ; Dae, Nylin Kim. / Use of hydrogel microstructures as templates for protein immobilization. Responsive Biomaterials for Biomedical Applications. Vol. 1095 2008. pp. 60-65
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Koh, WG & Dae, NK 2008, Use of hydrogel microstructures as templates for protein immobilization. in Responsive Biomaterials for Biomedical Applications. vol. 1095, pp. 60-65, 2008 MRS Spring Meeting, San Francisco, CA, United States, 08/3/24.

Use of hydrogel microstructures as templates for protein immobilization. / Koh, Won G.; Dae, Nylin Kim.

Responsive Biomaterials for Biomedical Applications. Vol. 1095 2008. p. 60-65.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Koh WG, Dae NK. Use of hydrogel microstructures as templates for protein immobilization. In Responsive Biomaterials for Biomedical Applications. Vol. 1095. 2008. p. 60-65