Use of ion pairing reagents for sensitive detection and separation of phospholipids in the positive ion mode LC-ESI-MS

Edra Dodbiba, Chengdong Xu, Tharanga Payagala, Eranda Wanigasekara, Myeong Hee Moon, Daniel W. Armstrong

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Phospholipids make up one of the more important classes of biological molecules. Because of their amphipathic nature and their charge state (e.g., negatively charged or zwitterionic) detection of trace levels of these compounds can be problematic. Electrospray ionization mass spectrometry (ESI-MS) is used in this study to detect very small amounts of these analytes by using the positive ion mode and pairing them with fifteen different cationic ion pairing reagents. The phospholipids used in this analysis were phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), 1,2-diheptanoyl-sn- glycero-3-phosphocholine (DHPC), cardiolipin (CA) and sphingosyl phosphoethanolamine (SPE). The analysis of these molecules was carried out in the single ion monitoring (SIM) positive mode. In addition to their detection, a high performance liquid chromatography and mass spectrometry (HPLC-MS) method was developed in which the phospholipids were separated and detected simultaneously within a very short period of time. Separation of phospholipids was developed in the reverse phase mode and in the hydrophilic interaction liquid chromatography (HILIC) mode HPLC. Their differences and impact on the sensitivity of the analytes are compared and discussed further in the paper. With this technique, limits of detection (LODs) were very easily recorded at low ppt (ng L-1) levels with many of the cationic ion pairing reagents used in this study.

Original languageEnglish
Pages (from-to)1586-1593
Number of pages8
JournalAnalyst
Volume136
Issue number8
DOIs
Publication statusPublished - 2011 Apr 21

Fingerprint

Electrospray ionization
Electrospray Ionization Mass Spectrometry
Phospholipids
phospholipid
Mass spectrometry
ionization
mass spectrometry
Positive ions
Ions
ion
liquid chromatography
High Pressure Liquid Chromatography
Phosphatidylglycerols
Phosphatidic Acids
Molecules
Cardiolipins
Phosphorylcholine
Phosphatidylserines
Liquid chromatography
High performance liquid chromatography

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Dodbiba, Edra ; Xu, Chengdong ; Payagala, Tharanga ; Wanigasekara, Eranda ; Moon, Myeong Hee ; Armstrong, Daniel W. / Use of ion pairing reagents for sensitive detection and separation of phospholipids in the positive ion mode LC-ESI-MS. In: Analyst. 2011 ; Vol. 136, No. 8. pp. 1586-1593.
@article{fee3eb2c89be4d3cac5cb41628aebe54,
title = "Use of ion pairing reagents for sensitive detection and separation of phospholipids in the positive ion mode LC-ESI-MS",
abstract = "Phospholipids make up one of the more important classes of biological molecules. Because of their amphipathic nature and their charge state (e.g., negatively charged or zwitterionic) detection of trace levels of these compounds can be problematic. Electrospray ionization mass spectrometry (ESI-MS) is used in this study to detect very small amounts of these analytes by using the positive ion mode and pairing them with fifteen different cationic ion pairing reagents. The phospholipids used in this analysis were phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), 1,2-diheptanoyl-sn- glycero-3-phosphocholine (DHPC), cardiolipin (CA) and sphingosyl phosphoethanolamine (SPE). The analysis of these molecules was carried out in the single ion monitoring (SIM) positive mode. In addition to their detection, a high performance liquid chromatography and mass spectrometry (HPLC-MS) method was developed in which the phospholipids were separated and detected simultaneously within a very short period of time. Separation of phospholipids was developed in the reverse phase mode and in the hydrophilic interaction liquid chromatography (HILIC) mode HPLC. Their differences and impact on the sensitivity of the analytes are compared and discussed further in the paper. With this technique, limits of detection (LODs) were very easily recorded at low ppt (ng L-1) levels with many of the cationic ion pairing reagents used in this study.",
author = "Edra Dodbiba and Chengdong Xu and Tharanga Payagala and Eranda Wanigasekara and Moon, {Myeong Hee} and Armstrong, {Daniel W.}",
year = "2011",
month = "4",
day = "21",
doi = "10.1039/c0an00848f",
language = "English",
volume = "136",
pages = "1586--1593",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "8",

}

Use of ion pairing reagents for sensitive detection and separation of phospholipids in the positive ion mode LC-ESI-MS. / Dodbiba, Edra; Xu, Chengdong; Payagala, Tharanga; Wanigasekara, Eranda; Moon, Myeong Hee; Armstrong, Daniel W.

In: Analyst, Vol. 136, No. 8, 21.04.2011, p. 1586-1593.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of ion pairing reagents for sensitive detection and separation of phospholipids in the positive ion mode LC-ESI-MS

AU - Dodbiba, Edra

AU - Xu, Chengdong

AU - Payagala, Tharanga

AU - Wanigasekara, Eranda

AU - Moon, Myeong Hee

AU - Armstrong, Daniel W.

PY - 2011/4/21

Y1 - 2011/4/21

N2 - Phospholipids make up one of the more important classes of biological molecules. Because of their amphipathic nature and their charge state (e.g., negatively charged or zwitterionic) detection of trace levels of these compounds can be problematic. Electrospray ionization mass spectrometry (ESI-MS) is used in this study to detect very small amounts of these analytes by using the positive ion mode and pairing them with fifteen different cationic ion pairing reagents. The phospholipids used in this analysis were phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), 1,2-diheptanoyl-sn- glycero-3-phosphocholine (DHPC), cardiolipin (CA) and sphingosyl phosphoethanolamine (SPE). The analysis of these molecules was carried out in the single ion monitoring (SIM) positive mode. In addition to their detection, a high performance liquid chromatography and mass spectrometry (HPLC-MS) method was developed in which the phospholipids were separated and detected simultaneously within a very short period of time. Separation of phospholipids was developed in the reverse phase mode and in the hydrophilic interaction liquid chromatography (HILIC) mode HPLC. Their differences and impact on the sensitivity of the analytes are compared and discussed further in the paper. With this technique, limits of detection (LODs) were very easily recorded at low ppt (ng L-1) levels with many of the cationic ion pairing reagents used in this study.

AB - Phospholipids make up one of the more important classes of biological molecules. Because of their amphipathic nature and their charge state (e.g., negatively charged or zwitterionic) detection of trace levels of these compounds can be problematic. Electrospray ionization mass spectrometry (ESI-MS) is used in this study to detect very small amounts of these analytes by using the positive ion mode and pairing them with fifteen different cationic ion pairing reagents. The phospholipids used in this analysis were phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), 1,2-diheptanoyl-sn- glycero-3-phosphocholine (DHPC), cardiolipin (CA) and sphingosyl phosphoethanolamine (SPE). The analysis of these molecules was carried out in the single ion monitoring (SIM) positive mode. In addition to their detection, a high performance liquid chromatography and mass spectrometry (HPLC-MS) method was developed in which the phospholipids were separated and detected simultaneously within a very short period of time. Separation of phospholipids was developed in the reverse phase mode and in the hydrophilic interaction liquid chromatography (HILIC) mode HPLC. Their differences and impact on the sensitivity of the analytes are compared and discussed further in the paper. With this technique, limits of detection (LODs) were very easily recorded at low ppt (ng L-1) levels with many of the cationic ion pairing reagents used in this study.

UR - http://www.scopus.com/inward/record.url?scp=79953230167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953230167&partnerID=8YFLogxK

U2 - 10.1039/c0an00848f

DO - 10.1039/c0an00848f

M3 - Article

C2 - 21336348

AN - SCOPUS:79953230167

VL - 136

SP - 1586

EP - 1593

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 8

ER -