Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis

TaeHyun Yoo, Hee Chang Kyung, Dong Ryeol Ryu, Sung Kim Ju, Young Choi Hoon, Cheon Park Hyeong, Shin-Wook Kang, Hun Choi Kyu, Myung Kim June, Kyu Ha Sung, Suk Han Dae, Yung Lee Ho

Research output: Contribution to journalArticle

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Abstract

Background: Peritonitis is the most common complication of continuous ambulatory peritonea I dialysis (CAPD), and the spectrum of organisms causing CAPD peritonitis is broad. Polymerase chain reaction (PCR) has recently broadened its diagnostic capabilities in infectious diseases. PCR can provide a sensitive method for identifying causative infectious organisms. Methods: To evaluate the usefulness of 23S bacterial ribosomal RNA amplification and direct sequencing for the detection of infectious organisms, we compared PCR with bacteriological culture for the analysis of dialysates from CAPD peritonitis patients. Thirty-two samples from CAPD peritonitis patients with current antibiotic use and control samples from 30 CAPD patients without peritonitis were examined by PCR with sequencing analysis and by conventional bacteriological culture. In addition, 95 culture-positive samples and 39 culture-negative samples from CAPD peritonitis patients before antibiotic treatment were analyzed by PCR assay. Results: In the control samples from patients without CAPD peritonitis, false-positive rates were relatively rare: 3 of 30 in the PCR study and 2 of 30 in the culture study. Of the 134 CAPD peritonitis samples collected before antibiotic therapy, positive cultures were obtained in 70.9% (95/134) of them. In 75 of the culture-positive samples, the same microorganisms were confirmed by PCR assay, and the others showed discrepant results as compared with culture study. In 30 of the 39 culture-negative samples, microbial organisms were detected by PCR assay. Of the 32 samples from patients who developed CAPD peritonitis during antibiotic treatment, 17 (53.1%) were positive by PCR assay, and 5 (15.6%) were positive by culture. Conclusion: Our study suggests that broad-spectrum PCR with RNA sequencing can complement culture methods in the diagnosis of CAPD peritonitis, especially in patients with previous or current antibiotic use.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalAmerican Journal of Nephrology
Volume26
Issue number2
DOIs
Publication statusPublished - 2006 May 1

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Peritoneum
Peritonitis
Dialysis
Bacteria
Polymerase Chain Reaction
Anti-Bacterial Agents
23S Ribosomal RNA
Bacterial RNA
RNA Sequence Analysis
Dialysis Solutions
Communicable Diseases
Therapeutics

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Yoo, T., Kyung, H. C., Ryu, D. R., Ju, S. K., Hoon, Y. C., Hyeong, C. P., ... Ho, Y. L. (2006). Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis. American Journal of Nephrology, 26(2), 115-120. https://doi.org/10.1159/000092040
Yoo, TaeHyun ; Kyung, Hee Chang ; Ryu, Dong Ryeol ; Ju, Sung Kim ; Hoon, Young Choi ; Hyeong, Cheon Park ; Kang, Shin-Wook ; Kyu, Hun Choi ; June, Myung Kim ; Sung, Kyu Ha ; Dae, Suk Han ; Ho, Yung Lee. / Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis. In: American Journal of Nephrology. 2006 ; Vol. 26, No. 2. pp. 115-120.
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title = "Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis",
abstract = "Background: Peritonitis is the most common complication of continuous ambulatory peritonea I dialysis (CAPD), and the spectrum of organisms causing CAPD peritonitis is broad. Polymerase chain reaction (PCR) has recently broadened its diagnostic capabilities in infectious diseases. PCR can provide a sensitive method for identifying causative infectious organisms. Methods: To evaluate the usefulness of 23S bacterial ribosomal RNA amplification and direct sequencing for the detection of infectious organisms, we compared PCR with bacteriological culture for the analysis of dialysates from CAPD peritonitis patients. Thirty-two samples from CAPD peritonitis patients with current antibiotic use and control samples from 30 CAPD patients without peritonitis were examined by PCR with sequencing analysis and by conventional bacteriological culture. In addition, 95 culture-positive samples and 39 culture-negative samples from CAPD peritonitis patients before antibiotic treatment were analyzed by PCR assay. Results: In the control samples from patients without CAPD peritonitis, false-positive rates were relatively rare: 3 of 30 in the PCR study and 2 of 30 in the culture study. Of the 134 CAPD peritonitis samples collected before antibiotic therapy, positive cultures were obtained in 70.9{\%} (95/134) of them. In 75 of the culture-positive samples, the same microorganisms were confirmed by PCR assay, and the others showed discrepant results as compared with culture study. In 30 of the 39 culture-negative samples, microbial organisms were detected by PCR assay. Of the 32 samples from patients who developed CAPD peritonitis during antibiotic treatment, 17 (53.1{\%}) were positive by PCR assay, and 5 (15.6{\%}) were positive by culture. Conclusion: Our study suggests that broad-spectrum PCR with RNA sequencing can complement culture methods in the diagnosis of CAPD peritonitis, especially in patients with previous or current antibiotic use.",
author = "TaeHyun Yoo and Kyung, {Hee Chang} and Ryu, {Dong Ryeol} and Ju, {Sung Kim} and Hoon, {Young Choi} and Hyeong, {Cheon Park} and Shin-Wook Kang and Kyu, {Hun Choi} and June, {Myung Kim} and Sung, {Kyu Ha} and Dae, {Suk Han} and Ho, {Yung Lee}",
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Yoo, T, Kyung, HC, Ryu, DR, Ju, SK, Hoon, YC, Hyeong, CP, Kang, S-W, Kyu, HC, June, MK, Sung, KH, Dae, SH & Ho, YL 2006, 'Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis', American Journal of Nephrology, vol. 26, no. 2, pp. 115-120. https://doi.org/10.1159/000092040

Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis. / Yoo, TaeHyun; Kyung, Hee Chang; Ryu, Dong Ryeol; Ju, Sung Kim; Hoon, Young Choi; Hyeong, Cheon Park; Kang, Shin-Wook; Kyu, Hun Choi; June, Myung Kim; Sung, Kyu Ha; Dae, Suk Han; Ho, Yung Lee.

In: American Journal of Nephrology, Vol. 26, No. 2, 01.05.2006, p. 115-120.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Usefulness of 23S rRNA amplification by PCR in the detection of bacteria in CAPD peritonitis

AU - Yoo, TaeHyun

AU - Kyung, Hee Chang

AU - Ryu, Dong Ryeol

AU - Ju, Sung Kim

AU - Hoon, Young Choi

AU - Hyeong, Cheon Park

AU - Kang, Shin-Wook

AU - Kyu, Hun Choi

AU - June, Myung Kim

AU - Sung, Kyu Ha

AU - Dae, Suk Han

AU - Ho, Yung Lee

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Background: Peritonitis is the most common complication of continuous ambulatory peritonea I dialysis (CAPD), and the spectrum of organisms causing CAPD peritonitis is broad. Polymerase chain reaction (PCR) has recently broadened its diagnostic capabilities in infectious diseases. PCR can provide a sensitive method for identifying causative infectious organisms. Methods: To evaluate the usefulness of 23S bacterial ribosomal RNA amplification and direct sequencing for the detection of infectious organisms, we compared PCR with bacteriological culture for the analysis of dialysates from CAPD peritonitis patients. Thirty-two samples from CAPD peritonitis patients with current antibiotic use and control samples from 30 CAPD patients without peritonitis were examined by PCR with sequencing analysis and by conventional bacteriological culture. In addition, 95 culture-positive samples and 39 culture-negative samples from CAPD peritonitis patients before antibiotic treatment were analyzed by PCR assay. Results: In the control samples from patients without CAPD peritonitis, false-positive rates were relatively rare: 3 of 30 in the PCR study and 2 of 30 in the culture study. Of the 134 CAPD peritonitis samples collected before antibiotic therapy, positive cultures were obtained in 70.9% (95/134) of them. In 75 of the culture-positive samples, the same microorganisms were confirmed by PCR assay, and the others showed discrepant results as compared with culture study. In 30 of the 39 culture-negative samples, microbial organisms were detected by PCR assay. Of the 32 samples from patients who developed CAPD peritonitis during antibiotic treatment, 17 (53.1%) were positive by PCR assay, and 5 (15.6%) were positive by culture. Conclusion: Our study suggests that broad-spectrum PCR with RNA sequencing can complement culture methods in the diagnosis of CAPD peritonitis, especially in patients with previous or current antibiotic use.

AB - Background: Peritonitis is the most common complication of continuous ambulatory peritonea I dialysis (CAPD), and the spectrum of organisms causing CAPD peritonitis is broad. Polymerase chain reaction (PCR) has recently broadened its diagnostic capabilities in infectious diseases. PCR can provide a sensitive method for identifying causative infectious organisms. Methods: To evaluate the usefulness of 23S bacterial ribosomal RNA amplification and direct sequencing for the detection of infectious organisms, we compared PCR with bacteriological culture for the analysis of dialysates from CAPD peritonitis patients. Thirty-two samples from CAPD peritonitis patients with current antibiotic use and control samples from 30 CAPD patients without peritonitis were examined by PCR with sequencing analysis and by conventional bacteriological culture. In addition, 95 culture-positive samples and 39 culture-negative samples from CAPD peritonitis patients before antibiotic treatment were analyzed by PCR assay. Results: In the control samples from patients without CAPD peritonitis, false-positive rates were relatively rare: 3 of 30 in the PCR study and 2 of 30 in the culture study. Of the 134 CAPD peritonitis samples collected before antibiotic therapy, positive cultures were obtained in 70.9% (95/134) of them. In 75 of the culture-positive samples, the same microorganisms were confirmed by PCR assay, and the others showed discrepant results as compared with culture study. In 30 of the 39 culture-negative samples, microbial organisms were detected by PCR assay. Of the 32 samples from patients who developed CAPD peritonitis during antibiotic treatment, 17 (53.1%) were positive by PCR assay, and 5 (15.6%) were positive by culture. Conclusion: Our study suggests that broad-spectrum PCR with RNA sequencing can complement culture methods in the diagnosis of CAPD peritonitis, especially in patients with previous or current antibiotic use.

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