Using comparative genomics to understand molecular features of carbapenem-resistant Acinetobacter baumannii from South Korea causing invasive infections and their clinical implications

Min Hyung Kim, Haeyoung Jeong, Young Mi Sim, Soohyun Lee, Dongeun Yong, Choong Min Ryu, Jun Yong Choi

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6 Citations (Scopus)

Abstract

Acinetobacter baumannii is a highly potent nosocomial pathogen that is associated with increased in-hospital mortality. Here, we investigated the changes in molecular characteristics of carbapenem-resistant A. baumannii (CRAB) isolated from the blood samples of patients admitted to a tertiary hospital in South Korea from January 2009 to July 2015. Whole genome sequencing using the Illumina MiSeq platform and multi-locus sequence typing (MLST) were performed for 98 CRAB clinical isolates. In silico analyses for the prediction of antimicrobial resistance and virulence factor genes were performed. Plasmid sequences, including complete forms, were reconstructed from the sequence reads. Epidemiologic data were collected from the hospital database. MLST using the Oxford scheme revealed 10 sequence types of CRAB, of which ST191 was the dominant type (n = 59). Although blaOXA- 23 was shared by most analysed strains, the compositions of antimicrobial resistance determinants differed among sequence types. ST447 and ST451/ST1809 with a few resistance genes were isolated during the later years of the study period. The number of virulence genes increased, while that of ST191 did not change significantly over the investigation period. Intriguingly MLST types, compositions of antimicrobial resistance genes, and virulence genes had no association with clinical outcomes of CRAB bacteraemia. In conclusion, active changes in or accumulations of antimicrobial resistance determinants and virulence genes in CRAB were not observed during the research period. Molecular characteristics of CRAB had no association with clinical outcomes of CRAB bacteraemia.

Original languageEnglish
Article numbere0229416
JournalPloS one
Volume15
Issue number2
DOIs
Publication statusPublished - 2020 Feb 1

Bibliographical note

Funding Information:
This work was supported by Korea HIV/ AIDS Cohort Study (Grant No. 2019-ER5101-00), Ministry of Health & Welfare, South Korea (Grant No. HI14C1324), Ministry of Science, ICT & Future Planning, South Korea (Grant No. HGUARD_2013M3A6B2078952), a Severance Hospital Research fund for Clinical Excellence (SHRC) and the KRIBB initiative program. We would like to thank all of the nursing and laboratory staff as well as the physicians who supported this project. Finally, we give credit to all of the patients who took part in this study. We would also like to thank e-World Editing (https://eworldediting.com/index.php) for providing English language editing service.

Funding Information:
Funding:ThisworkwassupportedbyKoreaHIV/ AIDSCohortStudy(GrantNo.2019-ER5101-00), MinistryofHealth&Welfare,SouthKorea(Grant No.HI14C1324),MinistryofScience,ICT&Future Planning,SouthKorea(GrantNo.H- GUARD_2013M3A6B2078952),aSeverance

Publisher Copyright:
© 2020 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

All Science Journal Classification (ASJC) codes

  • General

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