Using FLIM in the study of permeability barrier function of aged and young skin

P. Xu, E. H. Choi, M. Q. Man, D. Crumrine, T. Mauro, P. Elias

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing. Endogenous Na +/H + antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
Publication statusPublished - 2006
EventMultiphoton Microscopy in the Biomedical Sciences VI - San Jose, CA, United States
Duration: 2006 Jan 222006 Jan 24

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6089
ISSN (Print)0277-786X

Other

OtherMultiphoton Microscopy in the Biomedical Sciences VI
CountryUnited States
CitySan Jose, CA
Period06/1/2206/1/24

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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