PC-12 cells originate from neuroblastic cells, which have an ability to differentiate into neuronlike cells. In this work, the purpose was to estimate the influence of microenvironments on cell attachment and neuritogenesis capacity of PC-12 cells on parylene-N and parylene-C films with and without ultraviolet (UV) light treatment. The estimate of total cell number after incubation for 72 h, the ratio of adherent to suspended cells, counting of neurite outgrowths on parylene-N or parylene-C films after UV exposure suggested that these films were suitable for proliferation as well as differentiation of PC-12 cells. The differences in surface properties of parylene-N and parylene-C films with and without UV exposure were analyzed by contact angle measurement, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy. According to these analyses, introduction of oxygen-related chemical functional groups was presumed to result in increased hydrophilicity and efficiency of protein immobilization on parylene-N and parylene-C films after UV treatment. According to fluorescent staining, western blotting, and cell cycle analysis, UV-treated parylene-C and parylene-N films appear to effectively facilitate simultaneous proliferation and differentiation of PC-12 cells with neurite outgrowth.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea (2012R1A2A2A03047461, 2014M3A9E5073818, 2011-0020285, and 2015R1D1A1A01057737) and was supported by the Incheon National University Research Grant in 2016. The sponsor had no role in study design; the collection, analysis, or interpretation of data; the writing of the report; or the decision to submit the article for publication.
© 2016 Elsevier Inc.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology