UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation

Won Taek Lee, Sung Ho Lee, Sean G. Carriedo, Rona G. Giffard, Yeon Jung Yoon, Ji Hyung Kim, Kyung Ah Park, Jong Eun Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.

Original languageEnglish
Pages (from-to)288-294
Number of pages7
JournalMolecules and cells
Volume14
Issue number2
Publication statusPublished - 2002 Oct 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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