Abstract
The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 288-294 |
| Number of pages | 7 |
| Journal | Molecules and Cells |
| Volume | 14 |
| Issue number | 2 |
| Publication status | Published - 2002 Oct 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Medicine(all)
- Molecular Biology
- Cell Biology
Cite this
}
UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation. / Lee, Won Taek; Lee, Sung Ho; Carriedo, Sean G.; Giffard, Rona G.; Yoon, Yeon Jung; Kim, Ji Hyung; Park, Kyung Ah; Lee, Jongeun.
In: Molecules and Cells, Vol. 14, No. 2, 01.10.2002, p. 288-294.Research output: Contribution to journal › Article
TY - JOUR
T1 - UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation
AU - Lee, Won Taek
AU - Lee, Sung Ho
AU - Carriedo, Sean G.
AU - Giffard, Rona G.
AU - Yoon, Yeon Jung
AU - Kim, Ji Hyung
AU - Park, Kyung Ah
AU - Lee, Jongeun
PY - 2002/10/1
Y1 - 2002/10/1
N2 - The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.
AB - The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=0037206509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037206509&partnerID=8YFLogxK
M3 - Article
C2 - 12442903
AN - SCOPUS:0037206509
VL - 14
SP - 288
EP - 294
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 2
ER -