UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation

Won Taek Lee, Sung Ho Lee, Sean G. Carriedo, Rona G. Giffard, Yeon Jung Yoon, Ji Hyung Kim, Kyung Ah Park, Jongeun Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90% after UV-irradiation while the depolarization in control cells was about 50%. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.

Original languageEnglish
Pages (from-to)288-294
Number of pages7
JournalMolecules and Cells
Volume14
Issue number2
Publication statusPublished - 2002 Oct 1

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Papillomaviridae
Mitochondrial Membranes
Caspase 3
Astrocytes
Apoptosis
Tumor Suppressor Protein p53
Mitochondrial Membrane Potential
DNA
Cell Death

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Lee, Won Taek ; Lee, Sung Ho ; Carriedo, Sean G. ; Giffard, Rona G. ; Yoon, Yeon Jung ; Kim, Ji Hyung ; Park, Kyung Ah ; Lee, Jongeun. / UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation. In: Molecules and Cells. 2002 ; Vol. 14, No. 2. pp. 288-294.
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abstract = "The human papilloma virus-type 16 (HPV-16) E6 and E7 proteins interact with the p53 and pRb tumor suppressor proteins, respectively. The effect of E6 or E7 expression on UV irradiation (5 and 20 J/m2)-induced genotoxic injury of confluent primary murine astrocytes was determined. Retroviral vectors were used to overexpress E6 and E7. Astrocytes expressing E7 showed increased vulnerability to UV-induced apoptosis while E6 over expressing astrocytes were protected from the same insults. Cell death in the E7 overexpressing cells was apoptotic because it showed DNA ladders, activation of caspase-3, formation of apoptotic bodies and decreased DNA content to less than the G0 level. After UV-irradiation the level of E2F1 in E7-expressing astrocytes was higher than E6-, LXSN- or mock-infected cells, and caspase-3 was activated to a greater extent. E7-expressing astrocytes showed the highest levels of Bax under normal growth conditions. The mitochondrial membrane potential of E7-expressing astrocytes was depolarized by 90{\%} after UV-irradiation while the depolarization in control cells was about 50{\%}. E6 overexpression decreased while E7 overexpression increased UV-induced astrocyte apoptosis.",
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UV-vulnerability of human papilloma virus type-16 E7-expressing astrocytes is associated with mitochondrial membrane depolarization and caspase-3 activation. / Lee, Won Taek; Lee, Sung Ho; Carriedo, Sean G.; Giffard, Rona G.; Yoon, Yeon Jung; Kim, Ji Hyung; Park, Kyung Ah; Lee, Jongeun.

In: Molecules and Cells, Vol. 14, No. 2, 01.10.2002, p. 288-294.

Research output: Contribution to journalArticle

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