Validation of gene expression changes of osteopontin and MMP-1 in primary and metastatic colorectal carcinomas

Junjeong Choi, Sangkyum Kim, Jeon Han Park, Namkyu Kim, Hoguen Kim

Research output: Contribution to journalArticle

Abstract

Background: Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited. Methods: Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays. Results: The expression of osteopontin was significantly higher in metastatic carcinoma than in primary carcinoma, as indicated by mRNA expression. The expression of MMP-1 was significantly lower in metastatic carcinoma. Expression of serpin A1 was not correlated with the microarray results. Conclusions: Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalKorean Journal of Pathology
Volume44
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

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Matrix Metalloproteinase 1
Osteopontin
Colorectal Neoplasms
Carcinoma
Gene Expression
alpha 1-Antitrypsin
Neoplasm Metastasis
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Colonic Neoplasms
Genes
Neoplasms
Immunohistochemistry
Messenger RNA
Survival

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

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title = "Validation of gene expression changes of osteopontin and MMP-1 in primary and metastatic colorectal carcinomas",
abstract = "Background: Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited. Methods: Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays. Results: The expression of osteopontin was significantly higher in metastatic carcinoma than in primary carcinoma, as indicated by mRNA expression. The expression of MMP-1 was significantly lower in metastatic carcinoma. Expression of serpin A1 was not correlated with the microarray results. Conclusions: Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.",
author = "Junjeong Choi and Sangkyum Kim and Park, {Jeon Han} and Namkyu Kim and Hoguen Kim",
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Validation of gene expression changes of osteopontin and MMP-1 in primary and metastatic colorectal carcinomas. / Choi, Junjeong; Kim, Sangkyum; Park, Jeon Han; Kim, Namkyu; Kim, Hoguen.

In: Korean Journal of Pathology, Vol. 44, No. 3, 01.06.2010, p. 225-233.

Research output: Contribution to journalArticle

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AU - Kim, Hoguen

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