Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components

Sung Ryeol Kim; Kyung Hee Park; Jae Hyun Lee; Bum Joon Kim; Jae Hwan Hwang; Kook Jin Lim; Jung Won Park

doi:10.4168/aair.2019.11.3.422

Validation of PROTIA™ allergy-Q 64 Atopy^® as a specific IgE measurement assay for 10 major allergen components

Sung Ryeol Kim, Kyung Hee Park, Jae Hyun Lee, Bum Joon Kim, Jae Hwan Hwang, Kook Jin Lim, Jung Won Park

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy^®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy^® and ImmunoCAP^® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy^®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP^®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy^® is reliable and comparable to the ImmunoCAP^® assay for component-resolved diagnosis.

Original language	English
Pages (from-to)	422-432
Number of pages	11
Journal	Allergy, Asthma and Immunology Research
Volume	11
Issue number	3
DOIs	https://doi.org/10.4168/aair.2019.11.3.422
Publication status	Published - 2019 May 1

Bibliographical note

Funding Information:
This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI13C0010).

Publisher Copyright:
Copyright © 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Pulmonary and Respiratory Medicine

Access to Document

10.4168/aair.2019.11.3.422

Cite this

@article{a2081d4f9b2f49868eab529577542a0c,

title = "Validation of PROTIA{\texttrademark} allergy-Q 64 Atopy{\textregistered} as a specific IgE measurement assay for 10 major allergen components",

abstract = "Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA{\texttrademark} Allergy-Q 64 Atopy{\textregistered}, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA{\texttrademark} Allergy-Q 64 Atopy{\textregistered} and ImmunoCAP{\textregistered} assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA{\texttrademark} Allergy-Q 64 Atopy{\textregistered}, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP{\textregistered}), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA{\texttrademark} Allergy-Q 64 Atopy{\textregistered} is reliable and comparable to the ImmunoCAP{\textregistered} assay for component-resolved diagnosis.",

author = "Kim, {Sung Ryeol} and Park, {Kyung Hee} and Lee, {Jae Hyun} and Kim, {Bum Joon} and Hwang, {Jae Hwan} and Lim, {Kook Jin} and Park, {Jung Won}",

note = "Funding Information: This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI13C0010). Publisher Copyright: Copyright {\textcopyright} 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.",

year = "2019",

month = may,

day = "1",

doi = "10.4168/aair.2019.11.3.422",

language = "English",

volume = "11",

pages = "422--432",

journal = "Allergy, Asthma and Immunology Research",

issn = "2092-7355",

publisher = "Korean Academy of Asthma, Allergy and Clinical Immunology",

number = "3",

}

TY - JOUR

T1 - Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components

AU - Kim, Sung Ryeol

AU - Park, Kyung Hee

AU - Lee, Jae Hyun

AU - Kim, Bum Joon

AU - Hwang, Jae Hwan

AU - Lim, Kook Jin

AU - Park, Jung Won

N1 - Funding Information: This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI13C0010). Publisher Copyright: Copyright © 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

AB - Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

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