Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components

Sung Ryeol Kim, Kyung Hee Park, Jae Hyun Lee, Bum Joon Kim, Jae Hwan Hwang, Kook Jin Lim, Jungwon Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

Original languageEnglish
Pages (from-to)422-432
Number of pages11
JournalAllergy, Asthma and Immunology Research
Volume11
Issue number3
DOIs
Publication statusPublished - 2019 May 1

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Allergens
Immunoglobulin E
Hypersensitivity
Dermatophagoides farinae
Dermatophagoides pteronyssinus
Gliadin
Lactalbumin
Lactoglobulins
Caseins
Dermatophagoides Antigens
Nonparametric Statistics
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Kim, Sung Ryeol ; Park, Kyung Hee ; Lee, Jae Hyun ; Kim, Bum Joon ; Hwang, Jae Hwan ; Lim, Kook Jin ; Park, Jungwon. / Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components. In: Allergy, Asthma and Immunology Research. 2019 ; Vol. 11, No. 3. pp. 422-432.
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abstract = "Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy{\circledR}, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy{\circledR} and ImmunoCAP{\circledR} assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy{\circledR}, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP{\circledR}), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88{\%} (group 1 HDM allergen, 100{\%}; group 2 HDM allergen, 94.6{\%}; Bet v 1, 97.4{\%}; Fel d 1, 90.5{\%}; Que a 1, 89.2{\%}; α-lactalbumin, 96{\%}; β-lactoglobulin, 88{\%}; casein, 88{\%}; ω-5 gliadin, 96{\%}; α-Gal, 100{\%}). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy{\circledR} is reliable and comparable to the ImmunoCAP{\circledR} assay for component-resolved diagnosis.",
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Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components. / Kim, Sung Ryeol; Park, Kyung Hee; Lee, Jae Hyun; Kim, Bum Joon; Hwang, Jae Hwan; Lim, Kook Jin; Park, Jungwon.

In: Allergy, Asthma and Immunology Research, Vol. 11, No. 3, 01.05.2019, p. 422-432.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Validation of PROTIA™ allergy-Q 64 Atopy® as a specific IgE measurement assay for 10 major allergen components

AU - Kim, Sung Ryeol

AU - Park, Kyung Hee

AU - Lee, Jae Hyun

AU - Kim, Bum Joon

AU - Hwang, Jae Hwan

AU - Lim, Kook Jin

AU - Park, Jungwon

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N2 - Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

AB - Purpose: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively measure sIgE to major allergen components. Methods: The sIgE detection by PROTIA™ Allergy-Q 64 Atopy® and ImmunoCAP® assays was compared using the sera of 125 Korean allergic patients. Group 1 and 2 allergens of house dust mites (HDMs; Dermatophagoides farinae (Der f) 1 and Der f 2 in PROTIA™ Allergy-Q 64 Atopy®, Dermatophagoides pteronyssinus (Der p) 1 and Der p 2 in ImmunoCAP®), Bet v 1, Fel d 1, Que a 1, ω-5 gliadin, α-lactalbumin, β-lactoglobulin, casein and α-Gal were measured by both assays. Results: Comparing the results from the 2 assays, the agreement rate for all the 10 allergens was > 88% (group 1 HDM allergen, 100%; group 2 HDM allergen, 94.6%; Bet v 1, 97.4%; Fel d 1, 90.5%; Que a 1, 89.2%; α-lactalbumin, 96%; β-lactoglobulin, 88%; casein, 88%; ω-5 gliadin, 96%; α-Gal, 100%). Correlation analysis indicated that, all the 10 allergen sIgEs showed more than moderate positive correlation (Pearson correlation coefficients > 0.640). Additionally, intra-class comparison showed more than high correlation for all the 10 allergens (Spearman's rank correlation coefficients > 0.743). Conclusions: PROTIA™ Allergy-Q 64 Atopy® is reliable and comparable to the ImmunoCAP® assay for component-resolved diagnosis.

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