Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells

Insu Park; Jong Won Lim; Sung Hoon Kim; Seungyeop Choi; Kwan Hwi Ko; Myung Gu Son; Woo Jin Chang; Young Ro Yoon; Sejung Yang; Jaehong Key; Yoon Suk Kim; Kilho Eom; Rashid Bashir; Sei Young Lee; Sang Woo Lee

doi:10.1021/acs.jpclett.0c01427

Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells

Insu Park, Jong Won Lim, Sung Hoon Kim, Seungyeop Choi, Kwan Hwi Ko, Myung Gu Son, Woo Jin Chang, Young Ro Yoon, Sejung Yang, Jaehong Key, Yoon Suk Kim, Kilho Eom, Rashid Bashir, Sei Young Lee, Sang Woo Lee

Division of Medical Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities. Herein, the variable dielectric polarization characteristics of cell membranes are reported. In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency. The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes. In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes. This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.

Original language	English
Pages (from-to)	7197-7203
Number of pages	7
Journal	Journal of Physical Chemistry Letters
Volume	11
Issue number	17
DOIs	https://doi.org/10.1021/acs.jpclett.0c01427
Publication status	Published - 2020 Sept 3

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea (NRF) grants (NRF-2017R1A2B2002076 and NRF-2016R1D1A1A02937019) and Brain Korea 21 Plus project (S20SR41D0101) funded by the Korean government.

Publisher Copyright:
© 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

Materials Science(all)
Physical and Theoretical Chemistry

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10.1021/acs.jpclett.0c01427

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title = "Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells",

abstract = "Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities. Herein, the variable dielectric polarization characteristics of cell membranes are reported. In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency. The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes. In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes. This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.",

author = "Insu Park and Lim, {Jong Won} and Kim, {Sung Hoon} and Seungyeop Choi and Ko, {Kwan Hwi} and Son, {Myung Gu} and Chang, {Woo Jin} and Yoon, {Young Ro} and Sejung Yang and Jaehong Key and Kim, {Yoon Suk} and Kilho Eom and Rashid Bashir and Lee, {Sei Young} and Lee, {Sang Woo}",

note = "Funding Information: This work was supported by National Research Foundation of Korea (NRF) grants (NRF-2017R1A2B2002076 and NRF-2016R1D1A1A02937019) and Brain Korea 21 Plus project (S20SR41D0101) funded by the Korean government. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = sep,

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doi = "10.1021/acs.jpclett.0c01427",

language = "English",

volume = "11",

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T1 - Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells

AU - Park, Insu

AU - Lim, Jong Won

AU - Kim, Sung Hoon

AU - Choi, Seungyeop

AU - Ko, Kwan Hwi

AU - Son, Myung Gu

AU - Chang, Woo Jin

AU - Yoon, Young Ro

AU - Yang, Sejung

AU - Key, Jaehong

AU - Kim, Yoon Suk

AU - Eom, Kilho

AU - Bashir, Rashid

AU - Lee, Sei Young

AU - Lee, Sang Woo

N1 - Funding Information: This work was supported by National Research Foundation of Korea (NRF) grants (NRF-2017R1A2B2002076 and NRF-2016R1D1A1A02937019) and Brain Korea 21 Plus project (S20SR41D0101) funded by the Korean government. Publisher Copyright: © 2020 American Chemical Society.

PY - 2020/9/3

Y1 - 2020/9/3

N2 - Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities. Herein, the variable dielectric polarization characteristics of cell membranes are reported. In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency. The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes. In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes. This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.

AB - Investigation of the dielectric properties of cell membranes plays an important role in understanding the biological activities that sustain cellular life and realize cellular functionalities. Herein, the variable dielectric polarization characteristics of cell membranes are reported. In controlling the dielectric polarization of a cell using dielectrophoresis force spectroscopy, different cellular crossover frequencies were observed by modulating both the direction and sweep rate of the frequency. The crossover frequencies were used for the extraction of the variable capacitance, which is involved in the dielectric polarization across the cell membranes. In addition, this variable phenomenon was investigated by examining cells whose membranes were cholesterol-depleted with methyl-β-cyclodextrin, which verified a strong correlation between the variable dielectric polarization characteristics and membrane composition changes. This study presented the dielectric polarization properties in live cells' membranes that can be modified by the regulation of external stimuli and provided a powerful platform to explore cellular membrane dielectric polarization.

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Variable Membrane Dielectric Polarization Characteristic in Individual Live Cells

Abstract

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