Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Kyeongah Kang, Dae Hyoun Lim, In Hong Choi, Taegyeong Kang, Kangtaek Lee, Eun Yi Moon, Young Yang, Myeong Sok Lee, Jong Seok Lim

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Abstract

Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials due to their antibacterial properties. In this study, we examined the effects of polyvinylpyrrolidone (PVP)-coated AgNPs (average size 2.3. nm) on angiogenesis in both an in vivo model and an in vitro endothelial cell line, SVEC4-10. Increased angiogenesis was detected around the injection site of AgNP-containing Matrigel in vivo. AgNPs also increased the infiltration of endothelial cells and the hemoglobin (Hb) content in AgNP-Matrigel plugs implanted into mice. AgNPs induced endothelial cell tube formation on growth factor-reduced Matrigel, production of reactive oxygen species (ROS), and production of angiogenic factors, such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), in SVEC4-10 cells. In addition, AgNPs promoted the activation of FAK, Akt, ERK1/2, and p38, which are all involved in VEGF receptor (VEGFR)-mediated signaling. Finally, AgNP-treated tumors caused angiogenesis around tumors in B16F10 melanomas after they were injected into mice, and the Hb concentration in the tumors increased in a concentration-dependent manner with AgNP treatment. Thus, our study suggests that exposure to AgNPs can cause angiogenesis through the production of angiogenic factors.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalToxicology Letters
Volume205
Issue number3
DOIs
Publication statusPublished - 2011 Sep 10

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All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Kang, K., Lim, D. H., Choi, I. H., Kang, T., Lee, K., Moon, E. Y., Yang, Y., Lee, M. S., & Lim, J. S. (2011). Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles. Toxicology Letters, 205(3), 227-234. https://doi.org/10.1016/j.toxlet.2011.05.1033