Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation

Juyoung Kwon, Jong Seung Lee, Jaejun Lee, Jukwan Na, Jaesuk Sung, Hyo Jung Lee, Hankyul Kwak, Eunji Cheong, Seung Woo Cho, Heon Jin Choi

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular electrical stimulation (ES) can provide electrical potential from outside the cell membrane, but it is often ineffective due to interference from external factors such as culture medium resistance and membrane capacitance. To address this, we developed a vertical nanowire electrode array (VNEA) to directly provide intracellular electrical potential and current to cells through nanoelectrodes. Using this approach, the cell membrane resistivity and capacitance could be excluded, allowing effective ES. Human fetal neural stem cells (hfNSCs) were cultured on the VNEA for intracellular ES. Combining the structural properties of VNEA and VNEA-mediated ES, transient nanoscale perforation of the electrode was induced, promoting cell penetration and delivering current to the cell. Intracellular ES using VNEA improved the neuronal differentiation of hfNSCs more effectively than extracellular ES and facilitated electrophysiological functional maturation of hfNSCs because of the enhanced voltage-dependent ion-channel activity. The results demonstrate that VNEA with advanced nanoelectrodes serves as a highly effective culture and stimulation platform for stem-cell neurogenesis.

Original languageEnglish
Pages (from-to)6343-6351
Number of pages9
JournalNano letters
Volume21
Issue number14
DOIs
Publication statusPublished - 2021 Jul 28

Bibliographical note

Funding Information:
This research was supported by the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2018M3C7A1024654) and the Korean government (MSIP) (2017R1A2B3011586). This work was supported by a grant (2017M3C7A1047659) from the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT (MSIT), Republic of Korea. This work was also supported by the Institute for Basic Science (IBS-R026-D1). This research was partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2019.

Publisher Copyright:
© 2021 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'Vertical Nanowire Electrode Array for Enhanced Neurogenesis of Human Neural Stem Cells via Intracellular Electrical Stimulation'. Together they form a unique fingerprint.

Cite this