Objective: We aimed to assess the therapeutic efficacy of differentially modified soluble receptor for advanced glycation end products (sRAGE) in vivo using vessel ultrasound sonography and to compare the sonography data with those from postmortem histomorphologic analyses to have a practical reference for future clinical applications. Methods: Vessel ultrasound sonography was performed in a sRAGE-treated rat carotid artery balloon injury model at different time points after the surgery, and therapeutic efficacy of different doses of sRAGE produced in Chinese hamster ovary cells and with different N-glycoform modifications were assessed. Results: Vessel ultrasound sonography found that sRAGE produced in Chinese hamster ovary cells with complex N-glycoform modifications is highly effective, and is consistent with our recent findings in the same model assessed with histology. We also found that sonography is less sensitive than histology when a higher dose of sRAGE is administered. Conclusions: Sonograph results are consistent with those obtained from histology; that is, sRAGE produced in Chinese hamster ovary cells has significantly higher efficacy than insect cell-originated sRAGE cells.
|Number of pages||6|
|Journal||Current Therapeutic Research - Clinical and Experimental|
|Publication status||Published - 2014 Dec 1|
Bibliographical noteFunding Information:
This work was supported by the intramural research program of the National Institute on Aging, National Institutes of Health (Hyun-Jin Tae, Natalia Petrashevskaya, Ismayil Ahmet, Mark I. Talan, Edward G. Lakatta, and Li Lin), by a Priority Research Centers Program grant from the National Research Foundation ( NRF-2009-0093812 ) funded by the Ministry of Science, Information and Communication Technology, & Future Planning in the Republic of Korea (Hyun-Jin Tae), and by a Korea Research Foundation grant ( KRF-2009-013-E00008 ) (Sungha Park). Li Lin, Natalia Petrashevskaya, Ismayil Ahmet , Hyun-Jin Tae, and MarkI. Talan designed the studies; Hyun-Jin Tae, Natalia Petrashevskaya, Ismayil Ahmet, Sungha Park performed the studies; Natalia Petrashevskaya, Ismayil Ahmet, Hyun-Jin Tae, MarkI. Talan, Li Lin and EdwardG. Lakatta interpreted data; Li Lin and Ismayil Ahmet wrote the manuscript. The authors thank Wen Wei for purification of recombinant sRAGE.
All Science Journal Classification (ASJC) codes
- Pharmacology (medical)