Viability of Cells in Cryopreserved Canine Cardiovascular Organs fof Transplantation

Jong Chul Park, Hak Joon Sung, Dong Hee Lee, Young Hwan Park, Bum Koo Cho, Hwal Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins - fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.

Original languageEnglish
Pages (from-to)556-562
Number of pages7
JournalYonsei medical journal
Volume41
Issue number5
DOIs
Publication statusPublished - 2000 Oct

Fingerprint

Organ Transplantation
Canidae
Cell Survival
Endothelial Cells
Veins
Cryopreservation
Arteries
Trachea
Anti-Bacterial Agents
Griffonia
Therapeutics
Tissue Survival
Propidium
Fluorescein
Flow Cytometry
Dogs
Staining and Labeling
Transplants
Temperature

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Park, Jong Chul ; Sung, Hak Joon ; Lee, Dong Hee ; Park, Young Hwan ; Cho, Bum Koo ; Suh, Hwal. / Viability of Cells in Cryopreserved Canine Cardiovascular Organs fof Transplantation. In: Yonsei medical journal. 2000 ; Vol. 41, No. 5. pp. 556-562.
@article{5755720478204bf2b00fa03158ae6f67,
title = "Viability of Cells in Cryopreserved Canine Cardiovascular Organs fof Transplantation",
abstract = "To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins - fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.",
author = "Park, {Jong Chul} and Sung, {Hak Joon} and Lee, {Dong Hee} and Park, {Young Hwan} and Cho, {Bum Koo} and Hwal Suh",
year = "2000",
month = "10",
doi = "10.3349/ymj.2000.41.5.556",
language = "English",
volume = "41",
pages = "556--562",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "5",

}

Viability of Cells in Cryopreserved Canine Cardiovascular Organs fof Transplantation. / Park, Jong Chul; Sung, Hak Joon; Lee, Dong Hee; Park, Young Hwan; Cho, Bum Koo; Suh, Hwal.

In: Yonsei medical journal, Vol. 41, No. 5, 10.2000, p. 556-562.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Viability of Cells in Cryopreserved Canine Cardiovascular Organs fof Transplantation

AU - Park, Jong Chul

AU - Sung, Hak Joon

AU - Lee, Dong Hee

AU - Park, Young Hwan

AU - Cho, Bum Koo

AU - Suh, Hwal

PY - 2000/10

Y1 - 2000/10

N2 - To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins - fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.

AB - To determine applicability of the cryopreservation procedure for vessel grafts, the viability of endothelial cells (ECs) among the whole cells in three kinds of organs artery, vein, trachea in mongrel dogs was evaluated on the basis of histological analysis. The Griffonia simplicifolia agglutins - fluorescein isothiocyanate (GSA-FITC) and propidium iodide (PI) double staining methods were combined with flow cytometry (FCM), which was able to simultaneously determine the viability of whole cells and ECs from the same tissue, were performed after harvesting, after antibiotic solution treatment, and after cryopreservation and thawing. In most cases, the viability of ECs is lower than that of whole cells from veins and arteries. The viability of whole cells in veins was maintained until the antibiotic solution treatment and then decreased significantly after cryopreservation and thawing, while the ECs began to decrease significantly after the antibiotic solution treatment and more markedly decreased after thawing. The viability of ECs and whole cells from arteries was similar to that of the veins' conditions. The viability of whole cells from the trachea decreased with a similar pattern to that of the ECs from vessels. In consideration of maintaining cell viability among the three kinds of organs, the viability of arteries was better than that of the others. The cells in the trachea demonstrated a lower viability than the vessels. The effect of antibiotic solution treatment on the reduction of cell viability depends on the treatment time and temperature.

UR - http://www.scopus.com/inward/record.url?scp=0034297021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034297021&partnerID=8YFLogxK

U2 - 10.3349/ymj.2000.41.5.556

DO - 10.3349/ymj.2000.41.5.556

M3 - Article

C2 - 11079614

AN - SCOPUS:0034297021

VL - 41

SP - 556

EP - 562

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 5

ER -