Abstract
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 µm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
| Original language | English |
|---|---|
| Pages (from-to) | 381-385 |
| Number of pages | 5 |
| Journal | Journal of microbiology and biotechnology |
| Volume | 25 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 Apr 7 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
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Viable bacterial cell patterning using a pulsed jet electrospray system. / Chong, Eui Seok; Hwang, Gi Byung; Kim, Kyoungtae; Lee, Im Soon; Han, Song Hee; Kim, Hyung Joo; Jung, Heehoon; Kim, Sung Jin; Il Jung, Hyo; Uk Lee, Byung.
In: Journal of microbiology and biotechnology, Vol. 25, No. 3, 07.04.2014, p. 381-385.Research output: Contribution to journal › Article
TY - JOUR
T1 - Viable bacterial cell patterning using a pulsed jet electrospray system
AU - Chong, Eui Seok
AU - Hwang, Gi Byung
AU - Kim, Kyoungtae
AU - Lee, Im Soon
AU - Han, Song Hee
AU - Kim, Hyung Joo
AU - Jung, Heehoon
AU - Kim, Sung Jin
AU - Il Jung, Hyo
AU - Uk Lee, Byung
PY - 2014/4/7
Y1 - 2014/4/7
N2 - In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 µm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
AB - In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 µm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
UR - http://www.scopus.com/inward/record.url?scp=84925351991&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925351991&partnerID=8YFLogxK
U2 - 10.4014/jmb.1401.01012
DO - 10.4014/jmb.1401.01012
M3 - Article
C2 - 24705873
AN - SCOPUS:84925351991
VL - 25
SP - 381
EP - 385
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 3
ER -