Viable bacterial cell patterning using a pulsed jet electrospray system

Eui Seok Chong, Gi Byung Hwang, Kyoungtae Kim, Im Soon Lee, Song Hee Han, Hyung Joo Kim, Heehoon Jung, Sung Jin Kim, Hyo Il Jung, Byung Uk Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 µm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalJournal of microbiology and biotechnology
Volume25
Issue number3
DOIs
Publication statusPublished - 2014 Apr 7

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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