Visceral adiposity and expression of clock genes in peripheral blood mononuclear cells: A pilot study

Sue Kim, Hye Sun Lee, Hyun Ki Park, John A. Linton, Ji Won Lee, Hyangkyu Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Increasing evidence suggests a close interrelationship between disrupted circadian rhythms and obesity and metabolic disturbances. In particular, abdominal obesity, which contributes to the pathogenesis of metabolic disease, is associated with disrupted clock gene expression. However, little is known about the relationship between clock gene expression and accurate computed tomography (CT)-based measurements of visceral adiposity. Therefore, we examined the relationship between expression of clock genes in peripheral blood mononuclear cells (PBMCs) with visceral and subcutaneous adiposity in 75 healthy overweight or obese individuals. PBMCs were obtained from blood samples collected at 8 AM, and gene expression was analyzed by real-time reverse transcription polymerase chain reaction. Visceral and subcutaneous adiposity were measured by CT. Our results showed that visceral fat area was significantly positively correlated with BMAL1 and CRY1 mRNA levels and significantly negatively correlated with CLOCK, PER2, PER3 and CRY2 mRNA levels. In contrast, subcutaneous fat area was not correlated with the expression of any of the clock genes analyzed. After adjusting for multiple variables, visceral fat area was significantly associated with the expression of BMAL1, PER2 and CRY1. Taken together, our results indicate that visceral adiposity, but not subcutaneous adiposity, correlates with expression of clock genes in PBMCs.

Original languageEnglish
Pages (from-to)1057-1066
Number of pages10
JournalChronobiology International
Volume34
Issue number8
DOIs
Publication statusPublished - 2017 Sep 14

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Adiposity
Blood Cells
Gene Expression
Intra-Abdominal Fat
Tomography
Messenger RNA
Abdominal Obesity
Subcutaneous Fat
Metabolic Diseases
Circadian Rhythm
Reverse Transcription
Obesity
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Kim, Sue ; Lee, Hye Sun ; Park, Hyun Ki ; Linton, John A. ; Lee, Ji Won ; Lee, Hyangkyu. / Visceral adiposity and expression of clock genes in peripheral blood mononuclear cells : A pilot study. In: Chronobiology International. 2017 ; Vol. 34, No. 8. pp. 1057-1066.
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Visceral adiposity and expression of clock genes in peripheral blood mononuclear cells : A pilot study. / Kim, Sue; Lee, Hye Sun; Park, Hyun Ki; Linton, John A.; Lee, Ji Won; Lee, Hyangkyu.

In: Chronobiology International, Vol. 34, No. 8, 14.09.2017, p. 1057-1066.

Research output: Contribution to journalArticle

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