Viscoelastic protection from endothelial damage by air bubbles

Eungkweon Kim, Stephen M. Cristol, Shin J. Kang, Henry F. Edelhauser, Hyung Lae Kim, Jae Bum Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: To determine whether viscoelastic materials effectively protect the corneal endothelium from air bubbles. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Human eye-bank and rabbit eyes had a standardized phacoemulsification procedure with or without viscoelastic material (Healon® [sodium hyaluronate 1.0%], Healon GV® [sodium hyaluronate 1.4%], or Viscoat® [chondroitin sulfate 4.0%-sodium hyaluronate 3.0%]). The integrity of the endothelium was examined after the procedure with F-actin staining and scanning electron microscopy. Rabbit eyes with and without viscoelastic material (Healon or Viscoat) had a standardized irrigation/aspiration (I/A) procedure. The mucinous layer of the endothelium was examined after the procedure with transmission electron microscopy. Results: In the phacoemulsification experiment without viscoelastic material, with Healon, and with Healon GV, the endothelium of human and rabbit corneas had many areas of cell loss in a pattern consistent with air-bubble damage. With Viscoat, endothelial cells remained intact. In the I/A experiment, the mucinous layer of Viscoat-exposed rabbit endothelium appeared thinner. In the same experiments without viscoelastic material or with Healon, the mucinous layer of the endothelium appeared normal. Conclusions: Viscoat effectively protected the endothelium from air-bubble damage. Viscoat appears to protect the endothelium by acting as a physical barrier. Its adherence is probably related to the way it interacts with the mucinous layer of the endothelium.

Original languageEnglish
Pages (from-to)1047-1053
Number of pages7
JournalJournal of Cataract and Refractive Surgery
Volume28
Issue number6
DOIs
Publication statusPublished - 2002 Jun 13

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Hyaluronic Acid
Endothelium
Air
Rabbits
Phacoemulsification
Eye Banks
Corneal Endothelium
Architectural Accessibility
Ophthalmology
Korea
sodium hyaluronate drug combination chondroitin sulfate
Transmission Electron Microscopy
Electron Scanning Microscopy
Cornea
Actins
Endothelial Cells
Medicine
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Surgery
  • Ophthalmology
  • Sensory Systems

Cite this

Kim, E., Cristol, S. M., Kang, S. J., Edelhauser, H. F., Kim, H. L., & Lee, J. B. (2002). Viscoelastic protection from endothelial damage by air bubbles. Journal of Cataract and Refractive Surgery, 28(6), 1047-1053. https://doi.org/10.1016/S0886-3350(01)01319-0
Kim, Eungkweon ; Cristol, Stephen M. ; Kang, Shin J. ; Edelhauser, Henry F. ; Kim, Hyung Lae ; Lee, Jae Bum. / Viscoelastic protection from endothelial damage by air bubbles. In: Journal of Cataract and Refractive Surgery. 2002 ; Vol. 28, No. 6. pp. 1047-1053.
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Viscoelastic protection from endothelial damage by air bubbles. / Kim, Eungkweon; Cristol, Stephen M.; Kang, Shin J.; Edelhauser, Henry F.; Kim, Hyung Lae; Lee, Jae Bum.

In: Journal of Cataract and Refractive Surgery, Vol. 28, No. 6, 13.06.2002, p. 1047-1053.

Research output: Contribution to journalArticle

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T1 - Viscoelastic protection from endothelial damage by air bubbles

AU - Kim, Eungkweon

AU - Cristol, Stephen M.

AU - Kang, Shin J.

AU - Edelhauser, Henry F.

AU - Kim, Hyung Lae

AU - Lee, Jae Bum

PY - 2002/6/13

Y1 - 2002/6/13

N2 - Purpose: To determine whether viscoelastic materials effectively protect the corneal endothelium from air bubbles. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Human eye-bank and rabbit eyes had a standardized phacoemulsification procedure with or without viscoelastic material (Healon® [sodium hyaluronate 1.0%], Healon GV® [sodium hyaluronate 1.4%], or Viscoat® [chondroitin sulfate 4.0%-sodium hyaluronate 3.0%]). The integrity of the endothelium was examined after the procedure with F-actin staining and scanning electron microscopy. Rabbit eyes with and without viscoelastic material (Healon or Viscoat) had a standardized irrigation/aspiration (I/A) procedure. The mucinous layer of the endothelium was examined after the procedure with transmission electron microscopy. Results: In the phacoemulsification experiment without viscoelastic material, with Healon, and with Healon GV, the endothelium of human and rabbit corneas had many areas of cell loss in a pattern consistent with air-bubble damage. With Viscoat, endothelial cells remained intact. In the I/A experiment, the mucinous layer of Viscoat-exposed rabbit endothelium appeared thinner. In the same experiments without viscoelastic material or with Healon, the mucinous layer of the endothelium appeared normal. Conclusions: Viscoat effectively protected the endothelium from air-bubble damage. Viscoat appears to protect the endothelium by acting as a physical barrier. Its adherence is probably related to the way it interacts with the mucinous layer of the endothelium.

AB - Purpose: To determine whether viscoelastic materials effectively protect the corneal endothelium from air bubbles. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Human eye-bank and rabbit eyes had a standardized phacoemulsification procedure with or without viscoelastic material (Healon® [sodium hyaluronate 1.0%], Healon GV® [sodium hyaluronate 1.4%], or Viscoat® [chondroitin sulfate 4.0%-sodium hyaluronate 3.0%]). The integrity of the endothelium was examined after the procedure with F-actin staining and scanning electron microscopy. Rabbit eyes with and without viscoelastic material (Healon or Viscoat) had a standardized irrigation/aspiration (I/A) procedure. The mucinous layer of the endothelium was examined after the procedure with transmission electron microscopy. Results: In the phacoemulsification experiment without viscoelastic material, with Healon, and with Healon GV, the endothelium of human and rabbit corneas had many areas of cell loss in a pattern consistent with air-bubble damage. With Viscoat, endothelial cells remained intact. In the I/A experiment, the mucinous layer of Viscoat-exposed rabbit endothelium appeared thinner. In the same experiments without viscoelastic material or with Healon, the mucinous layer of the endothelium appeared normal. Conclusions: Viscoat effectively protected the endothelium from air-bubble damage. Viscoat appears to protect the endothelium by acting as a physical barrier. Its adherence is probably related to the way it interacts with the mucinous layer of the endothelium.

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