Watch out for your TRP1 marker: The effect of TRP1 gene on the growth at high and low temperatures in budding yeast

Gang Leng, Kiwon Song

Research output: Contribution to journalComment/debate

1 Citation (Scopus)

Abstract

TRP1 is a frequently used auxotrophic marker for genetic modifications and selections in trp-budding yeast strains, including the commonly used wild-type strain W303a. However, we found that introduction of the TRP1 gene into a trp-strain significantly affected vegetative growth at low and high temperatures. Therefore, caution should be needed when working in a trp-background strain and using the TRP1 marker to study stress response phenotypes, particularly when analyzing temperature sensitivities.

Original languageEnglish
Article numberfnw093
JournalFEMS Microbiology Letters
Volume363
Issue number10
DOIs
Publication statusPublished - 2016 May 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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