Water-deprivation-induced expression of neuronal nitric oxide synthase in the hypothalamic paraventricular nucleus of rat

Vitaly Ryu, Jong Ho Lee, Won Um Ji, Bae Yoo Sang, Jisun Lee, Kwang Chul Chung, Won Jahng Jeong

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6 Citations (Scopus)

Abstract

This study was conducted to define the molecular mechanism by which dehydration induces expression of neuronal nitric oxide synthase (nNOS) in the hypothalamic paraventricular nucleus (PVN). Rats were deprived from water for 48 hr and then sacrificed immediately or 1 hr after ad libitum access to water. Another group of rats had free access to food and water and was included as euhydrate control group. The PVN sections fixed with 4% paraformaldehyde were processed for nNOS immunohistochemistry and NADPH-diaphorase (NADPH-d)/pCREB or NADPH-d/c-Fos double staining. nNOS-ir neurons significantly increased with water deprivation and decreased with rehydration, both in the posterior magnocellular (pM)-and the medial parvocellular (mP)-PVN. Most NADPH-d histostained neurons in the PVN appeared to exhibit pCREB-ir as well. Water deprivation markedly increased, and rehydration decreased, NADPH-d/pCREB neurons both in the pM- and in the mP-PVN. Gel shift assay demonstrated that dehydration may promote CREB binding to nNOS promoter in the PVN neurons. Significant amounts of NADPH-d-stained neurons in the PVN of water-deprived rats (67-68% in both the mP and the pM) exhibited c-Fos-ir. NADPH-d/c-Fos neurons in the pM-PVN were increased by water deprivation but not changed by rehydration. NADPH-d/c-Fos double-stained neurons in the mP-PVN did not significantly change depending on different water conditions. These results suggest that pCREB may play a role in dehydration-induced nNOS gene expression in the PVN neurons, and c-Fos might not be implicated in the regulatory pathway.

Original languageEnglish
Pages (from-to)1371-1379
Number of pages9
JournalJournal of Neuroscience Research
Volume86
Issue number6
DOIs
Publication statusPublished - 2008 May 1

Fingerprint

Water Deprivation
Nitric Oxide Synthase Type I
Paraventricular Hypothalamic Nucleus
NADPH Dehydrogenase
Neurons
Fluid Therapy
Dehydration
Water
Gels
Immunohistochemistry
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

Ryu, Vitaly ; Lee, Jong Ho ; Ji, Won Um ; Sang, Bae Yoo ; Lee, Jisun ; Chung, Kwang Chul ; Jeong, Won Jahng. / Water-deprivation-induced expression of neuronal nitric oxide synthase in the hypothalamic paraventricular nucleus of rat. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 6. pp. 1371-1379.
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abstract = "This study was conducted to define the molecular mechanism by which dehydration induces expression of neuronal nitric oxide synthase (nNOS) in the hypothalamic paraventricular nucleus (PVN). Rats were deprived from water for 48 hr and then sacrificed immediately or 1 hr after ad libitum access to water. Another group of rats had free access to food and water and was included as euhydrate control group. The PVN sections fixed with 4{\%} paraformaldehyde were processed for nNOS immunohistochemistry and NADPH-diaphorase (NADPH-d)/pCREB or NADPH-d/c-Fos double staining. nNOS-ir neurons significantly increased with water deprivation and decreased with rehydration, both in the posterior magnocellular (pM)-and the medial parvocellular (mP)-PVN. Most NADPH-d histostained neurons in the PVN appeared to exhibit pCREB-ir as well. Water deprivation markedly increased, and rehydration decreased, NADPH-d/pCREB neurons both in the pM- and in the mP-PVN. Gel shift assay demonstrated that dehydration may promote CREB binding to nNOS promoter in the PVN neurons. Significant amounts of NADPH-d-stained neurons in the PVN of water-deprived rats (67-68{\%} in both the mP and the pM) exhibited c-Fos-ir. NADPH-d/c-Fos neurons in the pM-PVN were increased by water deprivation but not changed by rehydration. NADPH-d/c-Fos double-stained neurons in the mP-PVN did not significantly change depending on different water conditions. These results suggest that pCREB may play a role in dehydration-induced nNOS gene expression in the PVN neurons, and c-Fos might not be implicated in the regulatory pathway.",
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Water-deprivation-induced expression of neuronal nitric oxide synthase in the hypothalamic paraventricular nucleus of rat. / Ryu, Vitaly; Lee, Jong Ho; Ji, Won Um; Sang, Bae Yoo; Lee, Jisun; Chung, Kwang Chul; Jeong, Won Jahng.

In: Journal of Neuroscience Research, Vol. 86, No. 6, 01.05.2008, p. 1371-1379.

Research output: Contribution to journalArticle

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