Wavefront corrected light sheet microscopy in turbid media

H. I.C. Dalgarno; T. Čižmár; T. Vettenburg; J. Nylk; F. J. Gunn-Moore; K. Dholakia

doi:10.1063/1.4710527

Wavefront corrected light sheet microscopy in turbid media

H. I.C. Dalgarno, T. Čižmár, T. Vettenburg, J. Nylk, F. J. Gunn-Moore, K. Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an optimal light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.

Original language	English
Article number	191108
Journal	Applied Physics Letters
Volume	100
Issue number	19
DOIs	https://doi.org/10.1063/1.4710527
Publication status	Published - 2012 May 7

Bibliographical note

Funding Information:
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, and SULSA. K.D. is a Royal Society Wolfson Merit Award holder. The authors thank Martin Ploschner for assistance with the Mie theory calculations, and Professor Gang Wang for his help in the early phases of this project.

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.4710527

Cite this

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abstract = "Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an optimal light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.",

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AU - Dalgarno, H. I.C.

AU - Čižmár, T.

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AU - Nylk, J.

AU - Gunn-Moore, F. J.

AU - Dholakia, K.

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AB - Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an optimal light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.

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Wavefront corrected light sheet microscopy in turbid media

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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