Weight-bearing activity impairs nuclear membrane and genome integrity via YAP activation in plantar melanoma

Jimyung Seo; Hyun Seok Kim; Kyoung Il Min; Changgon Kim; Yongsoo Kwon; Zhenlong Zheng; Yusung Kim; Hyung Soon Park; Young Seok Ju; Mi Ryung Roh; Kee Yang Chung; Joon Kim

doi:10.1038/s41467-022-29925-x

Weight-bearing activity impairs nuclear membrane and genome integrity via YAP activation in plantar melanoma

Jimyung Seo, Hyun Seok Kim, Kyoung Il Min, Changgon Kim, Yongsoo Kwon, Zhenlong Zheng, Yusung Kim, Hyung Soon Park, Young Seok Ju, Mi Ryung Roh, Kee Yang Chung, Joon Kim

Department of Dermatology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Acral melanoma commonly occurs in areas that are not exposed to much sunlight, such as the sole of the foot. Little is known about risk factors and mutational processes of plantar acral melanoma. Nuclear envelope rupture during interphase contributes to genome instability in cancer. Here, we show that the nuclear and micronuclear membranes of melanoma cells are frequently ruptured by macroscopic mechanical stress on the plantar surface due to weight-bearing activities. The marginal region of plantar melanoma nodules exhibits increased nuclear morphological abnormalities and collagen accumulations, and is more susceptible to mechanical stress than the tumor center. An increase in DNA damage coincides with nuclear membrane rupture in the tumor margin. Nuclear envelope integrity is compromised by the mechanosensitive transcriptional cofactor YAP activated in the tumor margin. Our results suggest a mutagenesis mechanism in melanoma and explain why plantar acral melanoma is frequent at higher mechanical stress points.

Original language	English
Article number	2214
Journal	Nature communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-29925-x
Publication status	Published - 2022 Dec

Bibliographical note

Funding Information:
We thank the members of the J.K. lab for critical discussions. We would also like to thank M.Y.K. for schematic drawings and Dr. Yongsuk Hur of the KAIST BioMedical Research Center for helping with the preparation and acquisition of TEM images. Funding: This study was supported by the National Research Foundation of Korea Grant funded by the Korean Ministry of Science and ICT: 2020R1A2C3007748 to J.K., 2020R1A2C1102987 to K.Y.C., and 2021R1A4A1032094 to J.K.

Publisher Copyright:
© 2022, The Author(s).

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/s41467-022-29925-x

Cite this

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title = "Weight-bearing activity impairs nuclear membrane and genome integrity via YAP activation in plantar melanoma",

abstract = "Acral melanoma commonly occurs in areas that are not exposed to much sunlight, such as the sole of the foot. Little is known about risk factors and mutational processes of plantar acral melanoma. Nuclear envelope rupture during interphase contributes to genome instability in cancer. Here, we show that the nuclear and micronuclear membranes of melanoma cells are frequently ruptured by macroscopic mechanical stress on the plantar surface due to weight-bearing activities. The marginal region of plantar melanoma nodules exhibits increased nuclear morphological abnormalities and collagen accumulations, and is more susceptible to mechanical stress than the tumor center. An increase in DNA damage coincides with nuclear membrane rupture in the tumor margin. Nuclear envelope integrity is compromised by the mechanosensitive transcriptional cofactor YAP activated in the tumor margin. Our results suggest a mutagenesis mechanism in melanoma and explain why plantar acral melanoma is frequent at higher mechanical stress points.",

author = "Jimyung Seo and Kim, {Hyun Seok} and Min, {Kyoung Il} and Changgon Kim and Yongsoo Kwon and Zhenlong Zheng and Yusung Kim and Park, {Hyung Soon} and Ju, {Young Seok} and Roh, {Mi Ryung} and Chung, {Kee Yang} and Joon Kim",

note = "Funding Information: We thank the members of the J.K. lab for critical discussions. We would also like to thank M.Y.K. for schematic drawings and Dr. Yongsuk Hur of the KAIST BioMedical Research Center for helping with the preparation and acquisition of TEM images. Funding: This study was supported by the National Research Foundation of Korea Grant funded by the Korean Ministry of Science and ICT: 2020R1A2C3007748 to J.K., 2020R1A2C1102987 to K.Y.C., and 2021R1A4A1032094 to J.K. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

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AU - Roh, Mi Ryung

AU - Chung, Kee Yang

AU - Kim, Joon

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N2 - Acral melanoma commonly occurs in areas that are not exposed to much sunlight, such as the sole of the foot. Little is known about risk factors and mutational processes of plantar acral melanoma. Nuclear envelope rupture during interphase contributes to genome instability in cancer. Here, we show that the nuclear and micronuclear membranes of melanoma cells are frequently ruptured by macroscopic mechanical stress on the plantar surface due to weight-bearing activities. The marginal region of plantar melanoma nodules exhibits increased nuclear morphological abnormalities and collagen accumulations, and is more susceptible to mechanical stress than the tumor center. An increase in DNA damage coincides with nuclear membrane rupture in the tumor margin. Nuclear envelope integrity is compromised by the mechanosensitive transcriptional cofactor YAP activated in the tumor margin. Our results suggest a mutagenesis mechanism in melanoma and explain why plantar acral melanoma is frequent at higher mechanical stress points.

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Weight-bearing activity impairs nuclear membrane and genome integrity via YAP activation in plantar melanoma

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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