Whole-blood interferon-gamma release assay for diagnosis of tuberculous lymphadenitis

YoungKeun Kim, Young Uh, Nam Seok Lee, Meeyon Cho, Minseob Eom, Hyo Youl Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tuberculosis remains a major problem for much of the world. Tuberculous lymphadenitis is the most common type of extrapulmonary tuberculosis, although a difficult invasive procedure is required for its diagnosis. We evaluated the usefulness of the whole-blood interferon-gamma release assay (IGRA) for diagnosis of tuberculous lymphadenitis. From January 2008 to October 2010, 108 patients underwent lymph node biopsy and the IGRA concurrently in Wonju Christian Hospital, Yonsei University Wonju College of Medicine. Among the patients, 27 were diagnosed with tuberculous lymphadenitis and 81 were diagnosed with non-tuberculous lymphadenitis. The diagnostic performances of the IGRA were evaluated. The median patient age was 33 years (interquartile range [IQR] 23.5 to 48 years), and 28 (25.9%) patients were male. No patient was administered immunosuppressive agents such as high-dose steroids or underwent chemotherapy within 90 days before the IGRA test. The IGRA was positive in 25 of 27 patients with tuberculous lymphadenitis and in 13 of 81 patients with non-tuberculous lymphadenopathy. Therefore, the sensitivity of IGRA was 92.6% (95% CI, 82.0 to 100), and the specificity was 80.2% (95% CI, 71.4 to 89.1). In the patients with positive IGRA results, the INF-γ concentration was significantly higher in the patients with tuberculous lymphadenitis compared to that in the patients without tuberculous lymphadenitis (15.58 [IQR 6.87 to 45.10] IU/mL versus 0.97 [IQR 0.65 to 2.41] IU/mL, p < 0.001). In conclusion, the IGRA is helpful for the diagnosis of tuberculous lymphadenitis.

Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalTohoku Journal of Experimental Medicine
Volume224
Issue number3
DOIs
Publication statusPublished - 2011 Jul 1

Fingerprint

Interferon-gamma Release Tests
Lymph Node Tuberculosis
Interferon-gamma
Assays
Blood
Tuberculosis
Chemotherapy
Biopsy
Immunosuppressive Agents
Lymphadenitis
Medicine
Steroids
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kim, YoungKeun ; Uh, Young ; Lee, Nam Seok ; Cho, Meeyon ; Eom, Minseob ; Kim, Hyo Youl. / Whole-blood interferon-gamma release assay for diagnosis of tuberculous lymphadenitis. In: Tohoku Journal of Experimental Medicine. 2011 ; Vol. 224, No. 3. pp. 189-193.
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abstract = "Tuberculosis remains a major problem for much of the world. Tuberculous lymphadenitis is the most common type of extrapulmonary tuberculosis, although a difficult invasive procedure is required for its diagnosis. We evaluated the usefulness of the whole-blood interferon-gamma release assay (IGRA) for diagnosis of tuberculous lymphadenitis. From January 2008 to October 2010, 108 patients underwent lymph node biopsy and the IGRA concurrently in Wonju Christian Hospital, Yonsei University Wonju College of Medicine. Among the patients, 27 were diagnosed with tuberculous lymphadenitis and 81 were diagnosed with non-tuberculous lymphadenitis. The diagnostic performances of the IGRA were evaluated. The median patient age was 33 years (interquartile range [IQR] 23.5 to 48 years), and 28 (25.9{\%}) patients were male. No patient was administered immunosuppressive agents such as high-dose steroids or underwent chemotherapy within 90 days before the IGRA test. The IGRA was positive in 25 of 27 patients with tuberculous lymphadenitis and in 13 of 81 patients with non-tuberculous lymphadenopathy. Therefore, the sensitivity of IGRA was 92.6{\%} (95{\%} CI, 82.0 to 100), and the specificity was 80.2{\%} (95{\%} CI, 71.4 to 89.1). In the patients with positive IGRA results, the INF-γ concentration was significantly higher in the patients with tuberculous lymphadenitis compared to that in the patients without tuberculous lymphadenitis (15.58 [IQR 6.87 to 45.10] IU/mL versus 0.97 [IQR 0.65 to 2.41] IU/mL, p < 0.001). In conclusion, the IGRA is helpful for the diagnosis of tuberculous lymphadenitis.",
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Whole-blood interferon-gamma release assay for diagnosis of tuberculous lymphadenitis. / Kim, YoungKeun; Uh, Young; Lee, Nam Seok; Cho, Meeyon; Eom, Minseob; Kim, Hyo Youl.

In: Tohoku Journal of Experimental Medicine, Vol. 224, No. 3, 01.07.2011, p. 189-193.

Research output: Contribution to journalArticle

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