Wide-field multiphoton imaging through scattering media without correction

Adrià Escobet-Montalbán, Roman Spesyvtsev, Mingzhou Chen, Wardiya Afshar Saber, Melissa Andrews, C. Simon Herrington, Michael Mazilu, Kishan Dholakia

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard pointscanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.

Original languageEnglish
Article numberaau1338
JournalScience Advances
Volume4
Issue number10
DOIs
Publication statusPublished - 2018 Oct 12

All Science Journal Classification (ASJC) codes

  • General

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    Escobet-Montalbán, A., Spesyvtsev, R., Chen, M., Saber, W. A., Andrews, M., Simon Herrington, C., Mazilu, M., & Dholakia, K. (2018). Wide-field multiphoton imaging through scattering media without correction. Science Advances, 4(10), [aau1338]. https://doi.org/10.1126/sciadv.aau1338