Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán; Philip Wijesinghe; Mingzhou Chen; Kishan Dholakia

doi:10.1117/12.2508373

Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán, Philip Wijesinghe, Mingzhou Chen, Kishan Dholakia

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Optical approaches have broadened their impact in recent years with innovations in both wide-field and super- resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multi- photon images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while significantly reducing photobleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIX
Editors	Ammasi Periasamy, Peter T. C. So, Karsten Konig
Publisher	SPIE
ISBN (Electronic)	9781510624061
DOIs	https://doi.org/10.1117/12.2508373
Publication status	Published - 2019
Event	Multiphoton Microscopy in the Biomedical Sciences XIX 2019 - San Francisco, United States Duration: 2019 Feb 3 → 2019 Feb 6

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10882
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIX 2019
Country/Territory	United States
City	San Francisco
Period	19/2/3 → 19/2/6

Bibliographical note

Funding Information:
Funding: This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union’s Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512.

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2508373

Cite this

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title = "Wide-field multiphoton imaging with TRAFIX",

abstract = "Optical approaches have broadened their impact in recent years with innovations in both wide-field and super- resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multi- photon images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while significantly reducing photobleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.",

author = "Adri{\`a} Escobet-Montalb{\'a}n and Philip Wijesinghe and Mingzhou Chen and Kishan Dholakia",

note = "Funding Information: Funding: This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union{\textquoteright}s Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512. Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Multiphoton Microscopy in the Biomedical Sciences XIX 2019 ; Conference date: 03-02-2019 Through 06-02-2019",

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Escobet-Montalbán, A, Wijesinghe, P, Chen, M & Dholakia, K 2019, Wide-field multiphoton imaging with TRAFIX. in A Periasamy, PTC So & K Konig (eds), Multiphoton Microscopy in the Biomedical Sciences XIX., 108821G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10882, SPIE, Multiphoton Microscopy in the Biomedical Sciences XIX 2019, San Francisco, United States, 19/2/3. https://doi.org/10.1117/12.2508373

Wide-field multiphoton imaging with TRAFIX. / Escobet-Montalbán, Adrià; Wijesinghe, Philip; Chen, Mingzhou et al.

Multiphoton Microscopy in the Biomedical Sciences XIX. ed. / Ammasi Periasamy; Peter T. C. So; Karsten Konig. SPIE, 2019. 108821G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10882).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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Wide-field multiphoton imaging with TRAFIX

Abstract

Publication series

Conference

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

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