WNK1 promotes renal tumor progression by activating TRPC6-NFAT pathway

Ji Hee Kim, Kyu Hee Hwang, Minseob Eom, Minseon Kim, Eun Young Park, Yangsik Jeong, Kyusang Park, Seungkuy Cha

Research output: Contribution to journalArticle

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Abstract

Deregulation of Ca2+ signaling has been regarded as one of the key features of cancer progression. Lysine-deficient protein kinase 1 (WNK1), a major regulator of renal ion transport, regulates Ca2+ signaling through stimulating the phosphatidylinositol 4-kinase IIIα (PI4KIIIα) to activate Gαq-coupled receptor/PLC-β signaling. However, the contribution of WNK1-mediated Ca2+ signaling in the development of clear-cell renal-cell carcinoma (ccRCC) is yet unknown. We found that the canonical transient receptor potential channel (TRPC)6 was widely expressed in ccRCC tissues and functioned as a primary Ca2+ influx mechanism. We further identified that the expressions of WNK1, PI4KIIIα, TRPC6, and the nuclear factor of activated T cells cytoplasmic 1 (NFATc1) were elevated in the tumor tissues compared with the adjacent normal tissues. WNK1 expression was directly associated with the nuclear grade of ccRCC tissues. Functional experiments showed that WNK1 activated TRPC6-mediated Ca2+ influx and current by stimulating PI4KIIIα. Notably, the inhibition of WNK1-mediated TRPC6 activation and its downstream substrate calcineurin attenuated NFATc1 activation and the subsequent migration and proliferation of ccRCC. These findings revealed a novel perspective of WNK1 signaling in targeting the TRPC6-NFATc1 pathway as a therapeutic potential for renal-cell carcinoma.-Kim, J.-H., Hwang, K.-H., Eom, M., Kim, M., Park, E. Y., Jeong, Y., Park, K.-S., Cha, S.-K. WNK1 promotes renal tumor progression by activating TRPC6-NFAT pathway.

Original languageEnglish
Pages (from-to)8588-8599
Number of pages12
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

Fingerprint

Renal Cell Carcinoma
Tumors
NFATC Transcription Factors
1-Phosphatidylinositol 4-Kinase
Kidney
Tissue
Neoplasms
Chemical activation
Cells
Transient Receptor Potential Channels
Deregulation
Calcineurin
Ion Transport
Programmable logic controllers
Protein Kinases
Lysine
Ions
Substrates
Experiments
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Kim, Ji Hee ; Hwang, Kyu Hee ; Eom, Minseob ; Kim, Minseon ; Park, Eun Young ; Jeong, Yangsik ; Park, Kyusang ; Cha, Seungkuy. / WNK1 promotes renal tumor progression by activating TRPC6-NFAT pathway. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 7. pp. 8588-8599.
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WNK1 promotes renal tumor progression by activating TRPC6-NFAT pathway. / Kim, Ji Hee; Hwang, Kyu Hee; Eom, Minseob; Kim, Minseon; Park, Eun Young; Jeong, Yangsik; Park, Kyusang; Cha, Seungkuy.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 7, 01.07.2019, p. 8588-8599.

Research output: Contribution to journalArticle

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AB - Deregulation of Ca2+ signaling has been regarded as one of the key features of cancer progression. Lysine-deficient protein kinase 1 (WNK1), a major regulator of renal ion transport, regulates Ca2+ signaling through stimulating the phosphatidylinositol 4-kinase IIIα (PI4KIIIα) to activate Gαq-coupled receptor/PLC-β signaling. However, the contribution of WNK1-mediated Ca2+ signaling in the development of clear-cell renal-cell carcinoma (ccRCC) is yet unknown. We found that the canonical transient receptor potential channel (TRPC)6 was widely expressed in ccRCC tissues and functioned as a primary Ca2+ influx mechanism. We further identified that the expressions of WNK1, PI4KIIIα, TRPC6, and the nuclear factor of activated T cells cytoplasmic 1 (NFATc1) were elevated in the tumor tissues compared with the adjacent normal tissues. WNK1 expression was directly associated with the nuclear grade of ccRCC tissues. Functional experiments showed that WNK1 activated TRPC6-mediated Ca2+ influx and current by stimulating PI4KIIIα. Notably, the inhibition of WNK1-mediated TRPC6 activation and its downstream substrate calcineurin attenuated NFATc1 activation and the subsequent migration and proliferation of ccRCC. These findings revealed a novel perspective of WNK1 signaling in targeting the TRPC6-NFATc1 pathway as a therapeutic potential for renal-cell carcinoma.-Kim, J.-H., Hwang, K.-H., Eom, M., Kim, M., Park, E. Y., Jeong, Y., Park, K.-S., Cha, S.-K. WNK1 promotes renal tumor progression by activating TRPC6-NFAT pathway.

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