Abstract
Background/Aims To investigate the role of Wnt signalling in adipogenesis using an in vitro model of Graves' orbitopathy (GO). Methods Orbital fat was obtained from patients with GO and non-GO participants for primary orbital fibroblast (OF) culture. Expression levels of Wnt5a, Wnt10b, β-catenin, phospho-β-catenin and cyclin D1 were compared between GO and non-GO OFs. These expression levels were also determined during adipogenesis of GO and non-GO OFs. The effects of a stimulator and inhibitor of Wnt signalling on adipogenesis of GO and non-GO OFs were investigated. Results Western blotting analysis showed significant reductions in β-catenin and cyclin D1 and significant enhancement of phospho-β-catenin in OFs from patients with GO, compared with OFs from non-GO participants (p<0.05). Expression of Wnt5a, Wnt10b, β-catenin and cyclin D1 in OFs was highest on day 0, and then gradually declined after induction of adipogenic differentiation. The expression levels of PPAR 3, C/EBPα and C/EBPβ were reduced in Wnt stimulator-treated OFs in a dose-dependent manner. Oil red O staining confirmed that a stimulator of Wnt inhibited adipogenesis in GO OFs. Conclusion These results indicate that Wnt signalling inhibits adipogenesis in OFs from patients with GO and non-GO participants. Further studies are required to examine the potential of Wnt signalling as a target for therapeutic strategies.
Original language | English |
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Pages (from-to) | 1019-1027 |
Number of pages | 9 |
Journal | British Journal of Ophthalmology |
Volume | 106 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2022 Jul 1 |
Bibliographical note
Funding Information:Contributors SJJ, TKP and SYJ were responsible for the conception and design of the study, as well as the intellectual content. YJC performed experiments. SEW, BYK, J-SY and SYJ revised the article critically for intellectual content. All authors read and approved the final manuscript. Funding This work was supported by a National Research Foundation of Korea (NRF) grant funded by the government of Korea (MSIT) (No. 2020R1A2C4002095), and was partially supported by the Soonchunhyang University Research Fund.
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All Science Journal Classification (ASJC) codes
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience