Wound healing effect of adipose-derived stem cells: A critical role of secretory factors on human dermal fibroblasts

Won Serk Kim, Byung Soon Park, Jong Hyuk Sung, Jun Mo Yang, Seok Beom Park, Sahng June Kwak, Jeong Soo Park

Research output: Contribution to journalArticle

534 Citations (Scopus)

Abstract

Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells, which have characteristics similar to bone marrow-derived mesenchymal stem cells. Whereas ADSCs have potential applications for the repair and regeneration of various damaged tissues, few studies have dealt with the effect of ADSCs on fibroblasts, which play a key role in skin biology. Objective: In this study, we investigated the possible roles of ADSCs in skin wound healing process, especially in the aspect of fibroblast activation-proliferation, collagen synthesis and migratory properties. Methods and results: ADSCs promoted human dermal fibroblast (HDF) proliferation, not only by cell-to-cell direct contact, which was confirmed by co-culture experiment, but also by paracrine activation through secretory factors, resolved by transwell co-culture and culturing with conditioned medium of ADSCs (ADSC-CM). ADSC-CM enhanced the secretion of type I collagen in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins: up-regulation of collagen type I, III and fibronectin and down-regulation of MMP-1. Moreover, ADSC-CM showed stimulatory effect on migration of HDFs in in vitro wound healing models. Additional to those in vitro evidences, wound healing effect of ADSCs was also verified with in vivo animal study, resulted that ADSCs significantly reduced the wound size and accelerated the re-epithelialization from the edge. Conclusion: Collectively, these data suggest that ADSC is constitutionally well suited for dermal wound healing and secretory factors derived from ADSCs promote wound healing via HDFs and ADSCs can be used for the treatment of photoaging and wound healing.

Original languageEnglish
Pages (from-to)15-24
Number of pages10
JournalJournal of Dermatological Science
Volume48
Issue number1
DOIs
Publication statusPublished - 2007 Oct 1

Fingerprint

Fibroblasts
Stem cells
Wound Healing
Stem Cells
Skin
Coculture Techniques
Collagen Type I
Chemical activation
Re-Epithelialization
Collagen Type III
Extracellular Matrix Proteins
Conditioned Culture Medium
Matrix Metalloproteinases
Mesenchymal Stromal Cells
Fibronectins
Cell culture
Regeneration
Up-Regulation
Collagen
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Kim, Won Serk ; Park, Byung Soon ; Sung, Jong Hyuk ; Yang, Jun Mo ; Park, Seok Beom ; Kwak, Sahng June ; Park, Jeong Soo. / Wound healing effect of adipose-derived stem cells : A critical role of secretory factors on human dermal fibroblasts. In: Journal of Dermatological Science. 2007 ; Vol. 48, No. 1. pp. 15-24.
@article{f96393dc9bf54b059aa04ee0c34ce2c5,
title = "Wound healing effect of adipose-derived stem cells: A critical role of secretory factors on human dermal fibroblasts",
abstract = "Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells, which have characteristics similar to bone marrow-derived mesenchymal stem cells. Whereas ADSCs have potential applications for the repair and regeneration of various damaged tissues, few studies have dealt with the effect of ADSCs on fibroblasts, which play a key role in skin biology. Objective: In this study, we investigated the possible roles of ADSCs in skin wound healing process, especially in the aspect of fibroblast activation-proliferation, collagen synthesis and migratory properties. Methods and results: ADSCs promoted human dermal fibroblast (HDF) proliferation, not only by cell-to-cell direct contact, which was confirmed by co-culture experiment, but also by paracrine activation through secretory factors, resolved by transwell co-culture and culturing with conditioned medium of ADSCs (ADSC-CM). ADSC-CM enhanced the secretion of type I collagen in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins: up-regulation of collagen type I, III and fibronectin and down-regulation of MMP-1. Moreover, ADSC-CM showed stimulatory effect on migration of HDFs in in vitro wound healing models. Additional to those in vitro evidences, wound healing effect of ADSCs was also verified with in vivo animal study, resulted that ADSCs significantly reduced the wound size and accelerated the re-epithelialization from the edge. Conclusion: Collectively, these data suggest that ADSC is constitutionally well suited for dermal wound healing and secretory factors derived from ADSCs promote wound healing via HDFs and ADSCs can be used for the treatment of photoaging and wound healing.",
author = "Kim, {Won Serk} and Park, {Byung Soon} and Sung, {Jong Hyuk} and Yang, {Jun Mo} and Park, {Seok Beom} and Kwak, {Sahng June} and Park, {Jeong Soo}",
year = "2007",
month = "10",
day = "1",
doi = "10.1016/j.jdermsci.2007.05.018",
language = "English",
volume = "48",
pages = "15--24",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Wound healing effect of adipose-derived stem cells : A critical role of secretory factors on human dermal fibroblasts. / Kim, Won Serk; Park, Byung Soon; Sung, Jong Hyuk; Yang, Jun Mo; Park, Seok Beom; Kwak, Sahng June; Park, Jeong Soo.

In: Journal of Dermatological Science, Vol. 48, No. 1, 01.10.2007, p. 15-24.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Wound healing effect of adipose-derived stem cells

T2 - A critical role of secretory factors on human dermal fibroblasts

AU - Kim, Won Serk

AU - Park, Byung Soon

AU - Sung, Jong Hyuk

AU - Yang, Jun Mo

AU - Park, Seok Beom

AU - Kwak, Sahng June

AU - Park, Jeong Soo

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells, which have characteristics similar to bone marrow-derived mesenchymal stem cells. Whereas ADSCs have potential applications for the repair and regeneration of various damaged tissues, few studies have dealt with the effect of ADSCs on fibroblasts, which play a key role in skin biology. Objective: In this study, we investigated the possible roles of ADSCs in skin wound healing process, especially in the aspect of fibroblast activation-proliferation, collagen synthesis and migratory properties. Methods and results: ADSCs promoted human dermal fibroblast (HDF) proliferation, not only by cell-to-cell direct contact, which was confirmed by co-culture experiment, but also by paracrine activation through secretory factors, resolved by transwell co-culture and culturing with conditioned medium of ADSCs (ADSC-CM). ADSC-CM enhanced the secretion of type I collagen in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins: up-regulation of collagen type I, III and fibronectin and down-regulation of MMP-1. Moreover, ADSC-CM showed stimulatory effect on migration of HDFs in in vitro wound healing models. Additional to those in vitro evidences, wound healing effect of ADSCs was also verified with in vivo animal study, resulted that ADSCs significantly reduced the wound size and accelerated the re-epithelialization from the edge. Conclusion: Collectively, these data suggest that ADSC is constitutionally well suited for dermal wound healing and secretory factors derived from ADSCs promote wound healing via HDFs and ADSCs can be used for the treatment of photoaging and wound healing.

AB - Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells, which have characteristics similar to bone marrow-derived mesenchymal stem cells. Whereas ADSCs have potential applications for the repair and regeneration of various damaged tissues, few studies have dealt with the effect of ADSCs on fibroblasts, which play a key role in skin biology. Objective: In this study, we investigated the possible roles of ADSCs in skin wound healing process, especially in the aspect of fibroblast activation-proliferation, collagen synthesis and migratory properties. Methods and results: ADSCs promoted human dermal fibroblast (HDF) proliferation, not only by cell-to-cell direct contact, which was confirmed by co-culture experiment, but also by paracrine activation through secretory factors, resolved by transwell co-culture and culturing with conditioned medium of ADSCs (ADSC-CM). ADSC-CM enhanced the secretion of type I collagen in HDFs by regulating the mRNA levels of extracellular matrix (ECM) proteins: up-regulation of collagen type I, III and fibronectin and down-regulation of MMP-1. Moreover, ADSC-CM showed stimulatory effect on migration of HDFs in in vitro wound healing models. Additional to those in vitro evidences, wound healing effect of ADSCs was also verified with in vivo animal study, resulted that ADSCs significantly reduced the wound size and accelerated the re-epithelialization from the edge. Conclusion: Collectively, these data suggest that ADSC is constitutionally well suited for dermal wound healing and secretory factors derived from ADSCs promote wound healing via HDFs and ADSCs can be used for the treatment of photoaging and wound healing.

UR - http://www.scopus.com/inward/record.url?scp=34548017177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548017177&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2007.05.018

DO - 10.1016/j.jdermsci.2007.05.018

M3 - Article

C2 - 17643966

AN - SCOPUS:34548017177

VL - 48

SP - 15

EP - 24

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 1

ER -