Wound healing effect of slightly acidic electrolyzed water on cutaneous wounds in hairless mice via immune-redox modulation

Hae Sun You, Ailyn Fadriquela, Ma Easter Joy Sajo, Johny Bajgai, Jesmin Ara, Cheol Su Kim, Soo Ki Kim, Jin Rok Oh, Kwang Yong Shim, Hyun Kyo Lim, Kyu Jae Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Acidic electrolyzed water is an innovative sanitizer having a wide-spectrum of applications in food industry, and healthcare industry but little is known on its effect and mechanism in wound healing. The study was conducted to identify the effect and mechanism of slightly acidic electrolyzed water (SAEW) on cutaneous wounds in hairless mice. SAEW (pH: 5-6.5, oxidation reduction potential: 800 mV, chlorine concentration: 25 ppm) was prepared through electrolysis of water and was applied to the wounds of hairless mice three times a day for seven days. Wound size, immune response and oxidative stress were explored and compared to conventional agents such as Betadine and alcohol. We found that SAEW-treated group showed the highest wound reduction percentage (p<0.01). Antioxidant activities such as glutathione peroxidase, catalase and myeloperoxidase activities of SAEW group surpassed the total reactive oxygen species in skin. Nuclear factor erythroid-2-related-factor-2 and aryl hydrocarbon receptor were upregulated in SAEW group. Further, SAEW recruited the production of intracellular calcium and promoted its utilization for faster healing. In line, SAEW treatment decreased pro-inflammatory cytokines [interleukin (IL)-1ß, IL-6, keratinocyte chemoattractant, and tumor necrosis factor-a] in serum. Other hallmarks of wound healing, matrixmetalloproteinases (MMP)1 and MMP9 were also upregulated. Collectively, our study indicates that SAEW is effective in wound healing of hairless mice via immune-redox modulation, and heals better/faster than conventional agents.

Original languageEnglish
Pages (from-to)1423-1431
Number of pages9
JournalBiological and Pharmaceutical Bulletin
Volume40
Issue number9
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Hairless Mouse
Wound Healing
Oxidation-Reduction
Skin
Water
Wounds and Injuries
Electrolysis
Povidone-Iodine
Aryl Hydrocarbon Receptors
Health Care Sector
Water Purification
Food Industry
Chlorine
Chemotactic Factors
Glutathione Peroxidase
Interleukin-1
Keratinocytes
Catalase
Peroxidase
Interleukin-6

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

You, Hae Sun ; Fadriquela, Ailyn ; Sajo, Ma Easter Joy ; Bajgai, Johny ; Ara, Jesmin ; Kim, Cheol Su ; Kim, Soo Ki ; Oh, Jin Rok ; Shim, Kwang Yong ; Lim, Hyun Kyo ; Lee, Kyu Jae. / Wound healing effect of slightly acidic electrolyzed water on cutaneous wounds in hairless mice via immune-redox modulation. In: Biological and Pharmaceutical Bulletin. 2017 ; Vol. 40, No. 9. pp. 1423-1431.
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abstract = "Acidic electrolyzed water is an innovative sanitizer having a wide-spectrum of applications in food industry, and healthcare industry but little is known on its effect and mechanism in wound healing. The study was conducted to identify the effect and mechanism of slightly acidic electrolyzed water (SAEW) on cutaneous wounds in hairless mice. SAEW (pH: 5-6.5, oxidation reduction potential: 800 mV, chlorine concentration: 25 ppm) was prepared through electrolysis of water and was applied to the wounds of hairless mice three times a day for seven days. Wound size, immune response and oxidative stress were explored and compared to conventional agents such as Betadine and alcohol. We found that SAEW-treated group showed the highest wound reduction percentage (p<0.01). Antioxidant activities such as glutathione peroxidase, catalase and myeloperoxidase activities of SAEW group surpassed the total reactive oxygen species in skin. Nuclear factor erythroid-2-related-factor-2 and aryl hydrocarbon receptor were upregulated in SAEW group. Further, SAEW recruited the production of intracellular calcium and promoted its utilization for faster healing. In line, SAEW treatment decreased pro-inflammatory cytokines [interleukin (IL)-1{\ss}, IL-6, keratinocyte chemoattractant, and tumor necrosis factor-a] in serum. Other hallmarks of wound healing, matrixmetalloproteinases (MMP)1 and MMP9 were also upregulated. Collectively, our study indicates that SAEW is effective in wound healing of hairless mice via immune-redox modulation, and heals better/faster than conventional agents.",
author = "You, {Hae Sun} and Ailyn Fadriquela and Sajo, {Ma Easter Joy} and Johny Bajgai and Jesmin Ara and Kim, {Cheol Su} and Kim, {Soo Ki} and Oh, {Jin Rok} and Shim, {Kwang Yong} and Lim, {Hyun Kyo} and Lee, {Kyu Jae}",
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You, HS, Fadriquela, A, Sajo, MEJ, Bajgai, J, Ara, J, Kim, CS, Kim, SK, Oh, JR, Shim, KY, Lim, HK & Lee, KJ 2017, 'Wound healing effect of slightly acidic electrolyzed water on cutaneous wounds in hairless mice via immune-redox modulation', Biological and Pharmaceutical Bulletin, vol. 40, no. 9, pp. 1423-1431. https://doi.org/10.1248/bpb.b17-00219

Wound healing effect of slightly acidic electrolyzed water on cutaneous wounds in hairless mice via immune-redox modulation. / You, Hae Sun; Fadriquela, Ailyn; Sajo, Ma Easter Joy; Bajgai, Johny; Ara, Jesmin; Kim, Cheol Su; Kim, Soo Ki; Oh, Jin Rok; Shim, Kwang Yong; Lim, Hyun Kyo; Lee, Kyu Jae.

In: Biological and Pharmaceutical Bulletin, Vol. 40, No. 9, 01.01.2017, p. 1423-1431.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Wound healing effect of slightly acidic electrolyzed water on cutaneous wounds in hairless mice via immune-redox modulation

AU - You, Hae Sun

AU - Fadriquela, Ailyn

AU - Sajo, Ma Easter Joy

AU - Bajgai, Johny

AU - Ara, Jesmin

AU - Kim, Cheol Su

AU - Kim, Soo Ki

AU - Oh, Jin Rok

AU - Shim, Kwang Yong

AU - Lim, Hyun Kyo

AU - Lee, Kyu Jae

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Acidic electrolyzed water is an innovative sanitizer having a wide-spectrum of applications in food industry, and healthcare industry but little is known on its effect and mechanism in wound healing. The study was conducted to identify the effect and mechanism of slightly acidic electrolyzed water (SAEW) on cutaneous wounds in hairless mice. SAEW (pH: 5-6.5, oxidation reduction potential: 800 mV, chlorine concentration: 25 ppm) was prepared through electrolysis of water and was applied to the wounds of hairless mice three times a day for seven days. Wound size, immune response and oxidative stress were explored and compared to conventional agents such as Betadine and alcohol. We found that SAEW-treated group showed the highest wound reduction percentage (p<0.01). Antioxidant activities such as glutathione peroxidase, catalase and myeloperoxidase activities of SAEW group surpassed the total reactive oxygen species in skin. Nuclear factor erythroid-2-related-factor-2 and aryl hydrocarbon receptor were upregulated in SAEW group. Further, SAEW recruited the production of intracellular calcium and promoted its utilization for faster healing. In line, SAEW treatment decreased pro-inflammatory cytokines [interleukin (IL)-1ß, IL-6, keratinocyte chemoattractant, and tumor necrosis factor-a] in serum. Other hallmarks of wound healing, matrixmetalloproteinases (MMP)1 and MMP9 were also upregulated. Collectively, our study indicates that SAEW is effective in wound healing of hairless mice via immune-redox modulation, and heals better/faster than conventional agents.

AB - Acidic electrolyzed water is an innovative sanitizer having a wide-spectrum of applications in food industry, and healthcare industry but little is known on its effect and mechanism in wound healing. The study was conducted to identify the effect and mechanism of slightly acidic electrolyzed water (SAEW) on cutaneous wounds in hairless mice. SAEW (pH: 5-6.5, oxidation reduction potential: 800 mV, chlorine concentration: 25 ppm) was prepared through electrolysis of water and was applied to the wounds of hairless mice three times a day for seven days. Wound size, immune response and oxidative stress were explored and compared to conventional agents such as Betadine and alcohol. We found that SAEW-treated group showed the highest wound reduction percentage (p<0.01). Antioxidant activities such as glutathione peroxidase, catalase and myeloperoxidase activities of SAEW group surpassed the total reactive oxygen species in skin. Nuclear factor erythroid-2-related-factor-2 and aryl hydrocarbon receptor were upregulated in SAEW group. Further, SAEW recruited the production of intracellular calcium and promoted its utilization for faster healing. In line, SAEW treatment decreased pro-inflammatory cytokines [interleukin (IL)-1ß, IL-6, keratinocyte chemoattractant, and tumor necrosis factor-a] in serum. Other hallmarks of wound healing, matrixmetalloproteinases (MMP)1 and MMP9 were also upregulated. Collectively, our study indicates that SAEW is effective in wound healing of hairless mice via immune-redox modulation, and heals better/faster than conventional agents.

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