XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis

Annette M. McGehee, Stephanie K. Dougan, Elizabeth J. Klemm, Guanghou Shui, Boyoun Park, You Me Kim, Nicki Watson, Markus R. Wenk, Hidde L. Ploegh, Chih Chi Andrew Hu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The accumulation of misfolded secreted IgM in the endoplasmic reticulum (ER) of X-box binding protein 1 (XBP-1)-deficient B cells has been held responsible for the inability of such cells to yield plasma cells, through the failure to mount a proper unfolded protein response. LPS-stimulated B cells incapable of secreting IgM still activate the XBP-1 axis normally, as follows: XBP-1 is turned on by cues that trigger differentiation and not in response to accumulation of unfolded IgM, but the impact of XBP-1 deficiency on glycoprotein folding and assembly has not been explored. The lack of XBP-1 compromised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free κ-chains. Although XBP-1 deficiency affects the synthesis of some ER chaperones, including protein disulfide isomerase, their steady state levels do not drop below the threshold required for proper assembly and maturation of the Igα/Igβ heterodimer and MHC molecules. Intracellular transport and surface display of integral membrane proteins are unaffected by XBP-1 deficiency. Given the fact that we failed to observe any defects in folding of a variety of glycoproteins, we looked for other means to explain the requirement for XBP-1 in plasma cell development. We observed significantly reduced levels of phosphatidylcholine, sphingomyelin, and phosphatidylinositol in total membranes of XBP-1-deficient B cells, and reduced ER content. Terminal N-linked glycosylation of IgM and class I MHC was altered in these cells. XBP-1 hence has important roles beyond folding proteins in the ER.

Original languageEnglish
Pages (from-to)3690-3699
Number of pages10
JournalJournal of Immunology
Volume183
Issue number6
DOIs
Publication statusPublished - 2009 Sep 15

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Protein Folding
Glycosylation
Lipids
Immunoglobulin M
Protein Deficiency
Endoplasmic Reticulum
B-Lymphocytes
Plasma Cells
Glycoproteins
X-Box Binding Protein 1
Protein Disulfide-Isomerases
Unfolded Protein Response
Sphingomyelins
Phosphatidylinositols
Phosphatidylcholines
Cues
Membrane Proteins
Membranes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

McGehee, Annette M. ; Dougan, Stephanie K. ; Klemm, Elizabeth J. ; Shui, Guanghou ; Park, Boyoun ; Kim, You Me ; Watson, Nicki ; Wenk, Markus R. ; Ploegh, Hidde L. ; Hu, Chih Chi Andrew. / XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis. In: Journal of Immunology. 2009 ; Vol. 183, No. 6. pp. 3690-3699.
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abstract = "The accumulation of misfolded secreted IgM in the endoplasmic reticulum (ER) of X-box binding protein 1 (XBP-1)-deficient B cells has been held responsible for the inability of such cells to yield plasma cells, through the failure to mount a proper unfolded protein response. LPS-stimulated B cells incapable of secreting IgM still activate the XBP-1 axis normally, as follows: XBP-1 is turned on by cues that trigger differentiation and not in response to accumulation of unfolded IgM, but the impact of XBP-1 deficiency on glycoprotein folding and assembly has not been explored. The lack of XBP-1 compromised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free κ-chains. Although XBP-1 deficiency affects the synthesis of some ER chaperones, including protein disulfide isomerase, their steady state levels do not drop below the threshold required for proper assembly and maturation of the Igα/Igβ heterodimer and MHC molecules. Intracellular transport and surface display of integral membrane proteins are unaffected by XBP-1 deficiency. Given the fact that we failed to observe any defects in folding of a variety of glycoproteins, we looked for other means to explain the requirement for XBP-1 in plasma cell development. We observed significantly reduced levels of phosphatidylcholine, sphingomyelin, and phosphatidylinositol in total membranes of XBP-1-deficient B cells, and reduced ER content. Terminal N-linked glycosylation of IgM and class I MHC was altered in these cells. XBP-1 hence has important roles beyond folding proteins in the ER.",
author = "McGehee, {Annette M.} and Dougan, {Stephanie K.} and Klemm, {Elizabeth J.} and Guanghou Shui and Boyoun Park and Kim, {You Me} and Nicki Watson and Wenk, {Markus R.} and Ploegh, {Hidde L.} and Hu, {Chih Chi Andrew}",
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McGehee, AM, Dougan, SK, Klemm, EJ, Shui, G, Park, B, Kim, YM, Watson, N, Wenk, MR, Ploegh, HL & Hu, CCA 2009, 'XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis', Journal of Immunology, vol. 183, no. 6, pp. 3690-3699. https://doi.org/10.4049/jimmunol.0900953

XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis. / McGehee, Annette M.; Dougan, Stephanie K.; Klemm, Elizabeth J.; Shui, Guanghou; Park, Boyoun; Kim, You Me; Watson, Nicki; Wenk, Markus R.; Ploegh, Hidde L.; Hu, Chih Chi Andrew.

In: Journal of Immunology, Vol. 183, No. 6, 15.09.2009, p. 3690-3699.

Research output: Contribution to journalArticle

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T1 - XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis

AU - McGehee, Annette M.

AU - Dougan, Stephanie K.

AU - Klemm, Elizabeth J.

AU - Shui, Guanghou

AU - Park, Boyoun

AU - Kim, You Me

AU - Watson, Nicki

AU - Wenk, Markus R.

AU - Ploegh, Hidde L.

AU - Hu, Chih Chi Andrew

PY - 2009/9/15

Y1 - 2009/9/15

N2 - The accumulation of misfolded secreted IgM in the endoplasmic reticulum (ER) of X-box binding protein 1 (XBP-1)-deficient B cells has been held responsible for the inability of such cells to yield plasma cells, through the failure to mount a proper unfolded protein response. LPS-stimulated B cells incapable of secreting IgM still activate the XBP-1 axis normally, as follows: XBP-1 is turned on by cues that trigger differentiation and not in response to accumulation of unfolded IgM, but the impact of XBP-1 deficiency on glycoprotein folding and assembly has not been explored. The lack of XBP-1 compromised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free κ-chains. Although XBP-1 deficiency affects the synthesis of some ER chaperones, including protein disulfide isomerase, their steady state levels do not drop below the threshold required for proper assembly and maturation of the Igα/Igβ heterodimer and MHC molecules. Intracellular transport and surface display of integral membrane proteins are unaffected by XBP-1 deficiency. Given the fact that we failed to observe any defects in folding of a variety of glycoproteins, we looked for other means to explain the requirement for XBP-1 in plasma cell development. We observed significantly reduced levels of phosphatidylcholine, sphingomyelin, and phosphatidylinositol in total membranes of XBP-1-deficient B cells, and reduced ER content. Terminal N-linked glycosylation of IgM and class I MHC was altered in these cells. XBP-1 hence has important roles beyond folding proteins in the ER.

AB - The accumulation of misfolded secreted IgM in the endoplasmic reticulum (ER) of X-box binding protein 1 (XBP-1)-deficient B cells has been held responsible for the inability of such cells to yield plasma cells, through the failure to mount a proper unfolded protein response. LPS-stimulated B cells incapable of secreting IgM still activate the XBP-1 axis normally, as follows: XBP-1 is turned on by cues that trigger differentiation and not in response to accumulation of unfolded IgM, but the impact of XBP-1 deficiency on glycoprotein folding and assembly has not been explored. The lack of XBP-1 compromised neither the formation of functional hen egg lysozyme-specific IgM nor the secretion of free κ-chains. Although XBP-1 deficiency affects the synthesis of some ER chaperones, including protein disulfide isomerase, their steady state levels do not drop below the threshold required for proper assembly and maturation of the Igα/Igβ heterodimer and MHC molecules. Intracellular transport and surface display of integral membrane proteins are unaffected by XBP-1 deficiency. Given the fact that we failed to observe any defects in folding of a variety of glycoproteins, we looked for other means to explain the requirement for XBP-1 in plasma cell development. We observed significantly reduced levels of phosphatidylcholine, sphingomyelin, and phosphatidylinositol in total membranes of XBP-1-deficient B cells, and reduced ER content. Terminal N-linked glycosylation of IgM and class I MHC was altered in these cells. XBP-1 hence has important roles beyond folding proteins in the ER.

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