Xpert CARBA-R assay for the detection of carbapenemase-producing organisms in intensive care unit patients of a Korean tertiary care hospital

Do Kyun Kim, Hyoung Sun Kim, Naina Pinto, Jongsoo Jeon, Roshan D'Souza, Myung Sook Kim, Jun Yong Choi, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is a need to control CPO, especially in intensive care unit (ICU) patients, because these organisms are resistant to most β-lactam antibiotics and are easily transmitted. At present, the identification of CPO is time-consuming; hence, this study focused on the use of the Xpert CARBA-R assay (Cepheid, USA) to determine intestinal colonization rates of CPO in patients admitted to the ICU of a tertiary care hospital in Korea. Forty clinical stool samples were collected and inoculated both in a CARBA-R cartridge and in conventional culture plates. The CARBA-R assay required only ~one hour to screen CPO, while the time required for conventional culture was over three days. We also found that the prevalences of intestinal colonization by carbapenem-resistant organisms and Enterobacteriaceae were 17.5% (7 out of 40) and 7.5% (3 out of 40), respectively. Among the colonizing strains, three that contained carbapenemase, including Klebsiella pneumonia carbapenemase (KPC), and imipenem (IMP) and Verona integron-mediated metallo-β-lactamase (VIM) were found. With its convenience, the Xpert CARBA-R assay can be included in CPO surveillance strategies.

Original languageEnglish
Pages (from-to)162-165
Number of pages4
JournalAnnals of laboratory medicine
Volume36
Issue number2
DOIs
Publication statusPublished - 2016 Mar

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Intensive care units
Tertiary Healthcare
Tertiary Care Centers
Intensive Care Units
Assays
Integrons
Lactams
Carbapenems
carbapenemase
Imipenem
Klebsiella pneumoniae
Enterobacteriaceae
Korea
Cross Infection
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

@article{1a8d440b0f25491f87421a7e2c64b13f,
title = "Xpert CARBA-R assay for the detection of carbapenemase-producing organisms in intensive care unit patients of a Korean tertiary care hospital",
abstract = "Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is a need to control CPO, especially in intensive care unit (ICU) patients, because these organisms are resistant to most β-lactam antibiotics and are easily transmitted. At present, the identification of CPO is time-consuming; hence, this study focused on the use of the Xpert CARBA-R assay (Cepheid, USA) to determine intestinal colonization rates of CPO in patients admitted to the ICU of a tertiary care hospital in Korea. Forty clinical stool samples were collected and inoculated both in a CARBA-R cartridge and in conventional culture plates. The CARBA-R assay required only ~one hour to screen CPO, while the time required for conventional culture was over three days. We also found that the prevalences of intestinal colonization by carbapenem-resistant organisms and Enterobacteriaceae were 17.5{\%} (7 out of 40) and 7.5{\%} (3 out of 40), respectively. Among the colonizing strains, three that contained carbapenemase, including Klebsiella pneumonia carbapenemase (KPC), and imipenem (IMP) and Verona integron-mediated metallo-β-lactamase (VIM) were found. With its convenience, the Xpert CARBA-R assay can be included in CPO surveillance strategies.",
author = "Kim, {Do Kyun} and Kim, {Hyoung Sun} and Naina Pinto and Jongsoo Jeon and Roshan D'Souza and Kim, {Myung Sook} and Choi, {Jun Yong} and Dongeun Yong and Jeong, {Seok Hoon} and Kyungwon Lee",
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Xpert CARBA-R assay for the detection of carbapenemase-producing organisms in intensive care unit patients of a Korean tertiary care hospital. / Kim, Do Kyun; Kim, Hyoung Sun; Pinto, Naina; Jeon, Jongsoo; D'Souza, Roshan; Kim, Myung Sook; Choi, Jun Yong; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon.

In: Annals of laboratory medicine, Vol. 36, No. 2, 03.2016, p. 162-165.

Research output: Contribution to journalArticle

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T1 - Xpert CARBA-R assay for the detection of carbapenemase-producing organisms in intensive care unit patients of a Korean tertiary care hospital

AU - Kim, Do Kyun

AU - Kim, Hyoung Sun

AU - Pinto, Naina

AU - Jeon, Jongsoo

AU - D'Souza, Roshan

AU - Kim, Myung Sook

AU - Choi, Jun Yong

AU - Yong, Dongeun

AU - Jeong, Seok Hoon

AU - Lee, Kyungwon

PY - 2016/3

Y1 - 2016/3

N2 - Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is a need to control CPO, especially in intensive care unit (ICU) patients, because these organisms are resistant to most β-lactam antibiotics and are easily transmitted. At present, the identification of CPO is time-consuming; hence, this study focused on the use of the Xpert CARBA-R assay (Cepheid, USA) to determine intestinal colonization rates of CPO in patients admitted to the ICU of a tertiary care hospital in Korea. Forty clinical stool samples were collected and inoculated both in a CARBA-R cartridge and in conventional culture plates. The CARBA-R assay required only ~one hour to screen CPO, while the time required for conventional culture was over three days. We also found that the prevalences of intestinal colonization by carbapenem-resistant organisms and Enterobacteriaceae were 17.5% (7 out of 40) and 7.5% (3 out of 40), respectively. Among the colonizing strains, three that contained carbapenemase, including Klebsiella pneumonia carbapenemase (KPC), and imipenem (IMP) and Verona integron-mediated metallo-β-lactamase (VIM) were found. With its convenience, the Xpert CARBA-R assay can be included in CPO surveillance strategies.

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