Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme

Yang Li, Stefan Bjorklund, Yi Wei Jiang, Young Joon Kim, William S. Lane, David J. Stillman, Roger D. Kornberg

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Abstract

Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.

Original languageEnglish
Pages (from-to)10864-10868
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number24
DOIs
Publication statusPublished - 1995 Nov 21

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All Science Journal Classification (ASJC) codes

  • General

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