Abstract
Background: Serum chitinase-like proteins such as YKL-40 in asthmatic patients are known to positively correlate with disease severity but controversy remains regarding their role. The allergen bronchial provocation test (ABPT) can induce allergic airway inflammation in individuals with atopic asthma. Objective: To evaluate the induction and kinetics of YKL-40 during allergen-induced airway inflammation in atopic asthmatics. Methods: Thirteen patients were enrolled from May to November 2008. They all underwent ABPT with Dermatophagoides farinae crude extract. Induced sputums (IS) and serum were collected 3 times: 7 days before ABPT (baseline), 7 hours after ABPT, and 24 hours after ABPT. We examined the cytology of induced sputum (IS) and measured levels of YKL-40, interleukin (IL) 4, IL-5, IL-13, IL-33, tumor necrosis factor (TNF) a, and eosinophilic cationic protein (ECP) in IS and/or serum. Results: Following ABPT, total inflammatory cells, eosinophils, and neutrophils increased in a time-dependent manner in IS. YKL-40 levels were increased in IS but not in serum at 7 or 24 hours after ABPT (P=.011 and P=.006, respectively). Similarly to YKL-40, IL-5 and ECP levels were also increased in IS at 7 and 24 hours after ABPT (P=.011 for IL-5 and P=.006 for ECP). Overall, YKL-40 levels were well correlated with ECP levels in IS (p=0.576, P<.001). Conclusions: YKL-40 levels increased immediately in IS but not in the serum of atopic asthmatics. The correlation between YKL-40 levels and ECP in IS suggests that YKL-40 may play a pathophysiologic role in human atopic asthma.
| Original language | English |
|---|---|
| Pages (from-to) | 501-507 |
| Number of pages | 7 |
| Journal | Journal of Investigational Allergology and Clinical Immunology |
| Volume | 22 |
| Issue number | 7 |
| Publication status | Published - 2012 Dec 24 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
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YKL-40 in induced sputum after allergen bronchial provocation in atopic asthma. / Lee, J. H.; Park, K. H.; Park, Jungwon; Hong, C. S.
In: Journal of Investigational Allergology and Clinical Immunology, Vol. 22, No. 7, 24.12.2012, p. 501-507.Research output: Contribution to journal › Article
TY - JOUR
T1 - YKL-40 in induced sputum after allergen bronchial provocation in atopic asthma
AU - Lee, J. H.
AU - Park, K. H.
AU - Park, Jungwon
AU - Hong, C. S.
PY - 2012/12/24
Y1 - 2012/12/24
N2 - Background: Serum chitinase-like proteins such as YKL-40 in asthmatic patients are known to positively correlate with disease severity but controversy remains regarding their role. The allergen bronchial provocation test (ABPT) can induce allergic airway inflammation in individuals with atopic asthma. Objective: To evaluate the induction and kinetics of YKL-40 during allergen-induced airway inflammation in atopic asthmatics. Methods: Thirteen patients were enrolled from May to November 2008. They all underwent ABPT with Dermatophagoides farinae crude extract. Induced sputums (IS) and serum were collected 3 times: 7 days before ABPT (baseline), 7 hours after ABPT, and 24 hours after ABPT. We examined the cytology of induced sputum (IS) and measured levels of YKL-40, interleukin (IL) 4, IL-5, IL-13, IL-33, tumor necrosis factor (TNF) a, and eosinophilic cationic protein (ECP) in IS and/or serum. Results: Following ABPT, total inflammatory cells, eosinophils, and neutrophils increased in a time-dependent manner in IS. YKL-40 levels were increased in IS but not in serum at 7 or 24 hours after ABPT (P=.011 and P=.006, respectively). Similarly to YKL-40, IL-5 and ECP levels were also increased in IS at 7 and 24 hours after ABPT (P=.011 for IL-5 and P=.006 for ECP). Overall, YKL-40 levels were well correlated with ECP levels in IS (p=0.576, P<.001). Conclusions: YKL-40 levels increased immediately in IS but not in the serum of atopic asthmatics. The correlation between YKL-40 levels and ECP in IS suggests that YKL-40 may play a pathophysiologic role in human atopic asthma.
AB - Background: Serum chitinase-like proteins such as YKL-40 in asthmatic patients are known to positively correlate with disease severity but controversy remains regarding their role. The allergen bronchial provocation test (ABPT) can induce allergic airway inflammation in individuals with atopic asthma. Objective: To evaluate the induction and kinetics of YKL-40 during allergen-induced airway inflammation in atopic asthmatics. Methods: Thirteen patients were enrolled from May to November 2008. They all underwent ABPT with Dermatophagoides farinae crude extract. Induced sputums (IS) and serum were collected 3 times: 7 days before ABPT (baseline), 7 hours after ABPT, and 24 hours after ABPT. We examined the cytology of induced sputum (IS) and measured levels of YKL-40, interleukin (IL) 4, IL-5, IL-13, IL-33, tumor necrosis factor (TNF) a, and eosinophilic cationic protein (ECP) in IS and/or serum. Results: Following ABPT, total inflammatory cells, eosinophils, and neutrophils increased in a time-dependent manner in IS. YKL-40 levels were increased in IS but not in serum at 7 or 24 hours after ABPT (P=.011 and P=.006, respectively). Similarly to YKL-40, IL-5 and ECP levels were also increased in IS at 7 and 24 hours after ABPT (P=.011 for IL-5 and P=.006 for ECP). Overall, YKL-40 levels were well correlated with ECP levels in IS (p=0.576, P<.001). Conclusions: YKL-40 levels increased immediately in IS but not in the serum of atopic asthmatics. The correlation between YKL-40 levels and ECP in IS suggests that YKL-40 may play a pathophysiologic role in human atopic asthma.
UR - http://www.scopus.com/inward/record.url?scp=84871309020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871309020&partnerID=8YFLogxK
M3 - Article
C2 - 23397672
AN - SCOPUS:84871309020
VL - 22
SP - 501
EP - 507
JO - Journal of Investigational Allergology and Clinical Immunology
JF - Journal of Investigational Allergology and Clinical Immunology
SN - 1018-9068
IS - 7
ER -