Zizyphus jujuba and Codonopsis pilosula stimulate nitric oxide release in cultured endothelial cells and kidney tissues

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Abstract

Zizyphus jujuba and Codonopsis pilosula have been used for the treatment of nephritis in traditional oriental medicine. The present study aims to determine whether Zizyphus jujuba and Codonopsis pilosula stimulate NO releases in cultured endothelial cells and kidney tissues in vitro as well as in vivo by measuring the conversion of [14C]-L-arginine to [14C]-L-citrulline, a coproduct of the enzyme reaction from [14C]-L-arginine with NO. Confluent monolayer of endothelial cells and kidney homogenates (glomeruli, cortical tubules, medullae) were treated with Zizyphus jujuba and Codonopsis pilosula (final concentration 10 μg/ml) and NO releases were compared with those by receptor-dependent agonists, bradykinin and ADP and receptor-independent calcium ionophore A23187 in vitro. In rat experiment, NO releases in glomeruli, cortical tubules and medullae and plasma renin activity were assessed after intraperitoneal injection of Zizyphus jujuba and Codonopsis pilosula (10 mg/kg/day for 4 days). As a result, both Zizyphus jujuba and Codonopsis pilosula significantly increased NO releases in cultured endothelial cells, kidney tissues in vitro as well as in vivo. Stimulation of NO release by Zizyphus jujuba and Codonopsis pilosula was similar to those by receptor-dependent agonists, bradykinin and ADP in cultured endothelial cells. However, plasma renin activity was not influenced by these two natural products. In conclusion, stimulatory effects of Zizyphus jujuba and Codonopsis pilosula on NO release in kidney may contribute to their hypotensive effect and antinephritic action possibly by increasing renal blood flow.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalAsia Pacific Journal of Pharmacology
Volume11
Issue number3-4
Publication statusPublished - 1996 Dec 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology

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